The effect of Bo-hyul-an-shin-tang on the catecholamine excretion in rat urine under the cold environmental stress = 보혈안신탕이 한냉환경에서 흰쥐뇨증 Catecholamine 분비에 미치는 영향

국문 초록 (Abstract)

포유동물이 저온환경에 노출되면 두가지 방법으로 체열생산을 증가시킨다. 첫째는 신체적활동인 근육운동과 떨림이고 둘째는 hormone 생산의 증가이다. Davis와 Mayer는 쥐를 통한 실험에서 갑작스런 저온환경에서는 고온발생에 관여되는 화학적성분에 의하여 대사활동이 40% 증가한다고 발표하였다.
Hartman, Cannon등은 저온에 노출된 고양이에 있어서 Sympathoadreal activity가 증가하였다고 하였고 기타 여러학자들은 사람들이 저온에 노출되면 말초혈관축소, 혈압 상승등 교감신경 자극현상이 출현한다고 하였다. Von Euller는 몇 종류의 stress는 뇨증 catecholamine의 분리량을 증가시켰다고 한다.
저자는 시호, 백작약이 저온 sttess하에서 흰쥐의 뇨중 catecholamine의 분비량에 미치는 영향을 구명하였다.

다국어 초록 (Multilingual Abstract)

This research is focused on the variations of catecholamine secretion in rat urine which was collected in the cold envionment of 5℃.
One group of rat was administered Bo-hyul-an-shin-tang herb medicine extract and the 24 hour rat urine was extracted to take catecholamine by alumina adsorption method.
HPLC analysis were performed using a system incorporating a Varian Vista 5020(U.S.A) and Tacussels electrochemical detector(France), consisting of amperometric flow-cell with glassy carbon, stainless steel and Ag/AgCl electrode as working, auxilliary and reference electrode at the potential of +800 mV. Stainless Lichro-sorb, Rp 18, 10 um was used.
In the control group, the amount of norepinephrine was 190.2 ug/L, epinephrine was 126.6 ug/L, and dopamine was 63.1 ug/L. In Bohyulannshin-Tang administered group, norepinephrine was 126.6ug/L, epinephrine was 103.3ug/L, and dopamine was 32.6ug/L.
Authors could find the Be-hyul-an-shin-tang is effective in decrease of cafecholamine secretion to rat urine under the cold environment stress.

목차 (Table of Contents)

• Introduction
• Method
• Results and Discussion
• Conclusion