맥문동이 LPS로 유도된 폐손상에 미치는 영향

다국어 초록 (Multilingual Abstract)

This study was purposed to evaluate the effects of root of Liriope spicata (RLS) on LPS-induced COPD (chronic obstructive pulmonary disease) model. The extract of RLS was treated to A549 cells and LPS-induced COPD mice model. Then, various parameters such as cell-based cyto-protective activity and histopathological finding were analyzed. RLS showed a protective effect on LPS-induced cytotoxicity in A549 cells. This effect was correlated with analysis for caspase 3 levels, protein level of cyclin B1, Cdc2, and phospho-Erk1/2, and gene expression of TNF-α and IL-1β in A549 cells. RLS treatment also revealed the protective effect on LPS-induced lung injury in COPD mice model. This effect was evidenced via histopathological finding including immunofluence stains against caspase 3, and protein level of cyclin B1, Cdc2, and Erk1/2 in lung tissue. These data suggest that RLS has a pharmaceutical properties on lung injury. This study would provide an scientific evidence for the efficacy of RLS for clinical application to patients with COPD.

참고문헌 (Reference)

1 신정식, "한국산 맥문동과 중국산 소엽맥문동의 사포닌 성분" 한국작물학회 47 (47): 236-239, 2002

2 박시덕, "보음약인 사삼, 맥문동, 석곡, 옥죽, 황정의 면역조절 효과 비교" 대한동의병리학회 21 (21): 414-424, 2007

3 전성하, "맥문동이 생쥐의 기아 스트레스에 미치는 향" 대한한방내과학회 25 (25): 1-15, 2004

4 정해준, "맥문동(麥門冬)이 천식유발 cytokine 분비와 호산구 chemotaxis에 미치는 영향" 10 (10): 1-20, 2005

5 이숙경, "맥문동 열수추출물의 항산화력과 항균력에 관한 연구" 한국식품영양학회 22 (22): 279-285, 2009

6 이인자, "맥문동 열수추출물의 사염화탄소로 유발된 흰쥐의 간 손상에 대한 보호효과" 한국생약학회 23 (23): 166-171, 2003

7 이인자, "맥문동 엑스가 고콜레스테롤 식이를 급여한 흰쥐의 지질대사에 미치는 영향" 한국생약학회 34 (34): 65-69, 2003

8 임정교, "맥문동 물 추출물의 식이가 Streptozotocin으로 유도한 당뇨 흰쥐의 혈당과 혈청 콜레스테롤 함량에 미치는 영향" 동아시아식생활학회 15 (15): 20-28, 2005

9 조성지, "맥문동 (Liriope platyphylla W. T.) 스테로이드 사포닌의 항암활성" 41 (41): 390-394, 1998

10 유철규, "만성폐쇄성폐질환의 병인과 병태생리" 대한내과학회 77 (77): 383-400, 2009

11 장준, "만성 폐쇄성 폐질환의 약물치료" 대한결핵및호흡기학회 59 (59): 231-241, 2005

12 전국한의과대학 폐계내과학교실, "동의폐계내과학" 한문화사 335-346, 2002

13 김용현, "담배 연기에 의한 Muc5ac 유전자 발현에 관여하는 세포 내 신호 전달 경로로서의 ERF1/2와 p38 MAPR" 대한결핵및호흡기학회 58 (58): 590-599, 2005

14 이태희, "구속 스트레스로 유발된 기억 손상에 대한 麥門冬의 신경보호 효능" 대한본초학회 21 (21): 63-75, 2006

15 이병삼, "麥門冬이 C57BL/6J 생쥐의 Bleomycin 유발 肺纖維化에 미치는 影響" 대한한방내과학회 25 (25): 93-104, 2004

16 정해준, "麥門冬淸肺飮과 麥門冬이 인간기관지 상피세포의 IL-6, IL-16, GM-CSF mRNA level에 미치는 영향" 대한한의학회 23 (23): 11-23, 2002

17 전국한의과대학 본초학교실, "本草學" 영림사 588-589, 1994

18 장성은, "小葉麥門冬이 NC/Nga 아토피모델에 미치는 영향" 대한한방안이비인후피부과학회 21 (21): 10-19, 2008

19 오지석, "加味八味丸의 elastase 유도성 A549 세포사멸에 대한 보호효과" 대한한의학회 31 (31): 137-148, 2010

20 Back, N.I., "pesticide environment chemistry. Cytotoxicity of steroid saponins from the tuber of Liriope platyphylla W.T" 41 : 390-394, 1998

21 Tomoda, M., "Water Soluble carbohydrates of Ophiopogon tuber. Ⅱ, Purification, properties and structures of three oligosaccharides" 16 : 113-116, 1968

22 Nigg, E.A., "Vertebrate cdc2 kinase: its regulation by phosphorylation and its mitotic targets" 56 : 539-547, 1991

23 Whitmarsh, A.J., "Transcription factor AP-1 regulation by mitogen-activated protein kinase signal transduction pathways" 74 : 589-607, 1996

24 Lee, D.Y., "The new steroidal saponins from the tuber of Lirope spicata" 12 : 295-299, 1989

25 Draetta, G., "The mammalian cdc2 protein kinase: mechanisms of regulation during the cell cycle" 12 : 21-27, 1989

26 Curtis, J.L., "The immunopathogenesis of chronic obstructive pulmonary disease: insights from recent research" 4 : 512-521, 2007

27 Lee, D.J, "The effects of Lonicera japonica on the murine model of Chronic obstructive pulmonary disease" 경희대학교 2009

28 Weston, C.R., "The JNK signal transduction pathway" 12 : 14-21, 2002

29 Yanai, M., "Site of airway obstruction in pulmonary disease: direct measurement of intrabronchial pressure" 72 : 1016-1023, 1992

30 Kawakami, M., "Sequential and quantitative analysis of a murine model of elastase-induced emphysema" 31 : 1434-1438, 2008

31 Nakajoh, M., "Retinoic acid inhibits elastase-induced injury in human lung epithelial cell lines" 28 : 296-304, 2003

32 Nakajoh, M., "Retinoic acid inhibits elastase-induced injury in human lung epithelial cell lines" 28 : 296-304, 2003

33 Martin, T.R, "Recognition of bacterial endotoxin in the lungs" 23 : 128-132, 2000

34 Hogg, J.C, "Pathophysiology of airflow limitation in chronic obstructive pulmonary disease" 364 : 709-721, 2004

35 Xia, Z., "Opposing effects of ERK and JNK-p38 MAP kinases on apoptosis" 270 : 1326-1331, 1995

36 노성수, "NOD 당뇨병 생쥐에 미치는 맥문동의 항염증 효과" 대한동의병리학회 22 (22): 766-770, 2008

37 Segal, R.A., "Intracellular signaling pathways activated by neurotrophic factors" 19 : 463-489, 1996

38 Buist, A.S., "International variation in the prevalence of COPD (the BOLD Study): a population-based prevalence study" 370 (370): 741-750, 2007

39 Katrin Schuha, "Inhibition of the MAP kinase ERK protects from lipopolysaccharide-induced lung injury" 77 (77): 1827-1834, 2009

40 김지하, "HIT-T15 췌장세포의 인슐린분비 촉진을 유도하는 맥문동(Liriope platyphylla) 추출물의 효능 및 독성분석" 한국생명과학회 20 (20): 1027-1033, 2010

41 윤종만, "Elastase 매개성 폐조직 손상에 대한 宣肺定喘湯의 보호효과" 대한한방내과학회 31 (31): 84-101, 2010

42 Watanabe, Y., "Comparative studies on the constituents of Ophiopogon tuber and its congeners. Ⅲ. Studies on the constituents of the subterranean part of Ophiopogono ohwii and O. jaburan L" 32 : 3994-4002, 1984

43 Uhal, B.D, "Cell cycle kinetics in the alveolar epithelium" 272 : L1031-1045, 1997

44 Draetta, G, "Cell cycle control in eukaryotes: molecular mechanisms of cdc2 activation" 15 : 378-383, 1990

45 Grütter, M.G, "Caspases: key players in programmed cell death" 10 : 649-655, 2000

46 Flaherty, D.M., "Adenovirus vectors activate survival pathways in lung epithelial cells" 287 (287): L393-401, 2004