칸나에서 분리한 Cucumber mosaic virus의 특성|RISS 상세보기

다국어 초록 (Multilingual Abstract)

An isolate of Cucumber mosaic virus (CMV), called as Can-CMV, was originally isolated from Canna generalis
showing typical streak mosaic foliar symptoms, and its properties were investigated in this study. Whereas all
known isolates of CMV could induce symptoms on their systemic hosts (four kinds of Nicotiana spp and a
zucchini squash), Can-CMV induced no symptoms on its systemic hosts tested. Replication and movement of
the virus on upper leaves as well as inoculated leaves were confirmed by RT-PCR suggesting that Can-CMV
could only infect systemically on N. benthamiana and N. glutinosa. Size of local lesions on the Can-CMV- inoculated
leaves of Chenopodium amaranticolor was much smaller than that of Fny-CMV. Whereas Fny-CMV
and LS-CMV could induce distinct necrotic local lesions on Vigna unguiculata 2 to 3 days postinoculation
(dpi), chlorotic spots symptom was expressed by Can-CMV 4 to 5 dpi. Virus-specific 4 kinds of dsRNAs were
isolated from leaves of N. benthamiana infected with Can-CMV, and these dsRNAs corresponded to the viral
genomic RNAs and subgenomic RNAs and their patterns were indistinguishable to those of Fny-CMV and
LS-CMV. By restriction mapping analysis of 950 bp of RT-PCR amplified products of coat protein gene of the
virus as well as by serological analysis of gel diffusion test, Can-CMV belongs to a typical member of CMV
subgroup IA. These results suggest that the Can-CMV isolated from C. generalis possesses unique pathological
properties to understand further insight into the various interactions between virus and host.

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