In vivo Imaging of Escherichia coli and Lactococcus lactis in Murine Intestines Using a Reporter Luciferase Gene

다국어 초록 (Multilingual Abstract)

A recombinant DNA, pMG36e::luc+ (5.3 kb),was constructed and transferred to Escherichia coli MC1061 and Lactococcus lactis MG1363 for in vivo imaging of the bacteria in murine intestines. E. coli MC1061 (pMG36e::luc+) and L. lactis MG1363(pMG36e::luc+) displayed luciferase activities in vitro,where the bioluminescent signal of the E. coli was much stronger than that of the L. lactis by approximately 100-fold. These 2 recombinant bacteria were orally administered into rats. The bioluminescent signals of the E. coli and L.
lactis in the gastrointestinal (GI) tracts of rats were detected and maintained up to 2 h via whole body imaging,indicating that the luciferase gene expression system for bacteria could be applied for in vivo imaging.

참고문헌 (Reference)

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