The Korean isolates of porcine reproductive and respiratory syndrom(PRRS) virus, KPRRSV-1 and KPRRSV-2, were characterized on the aspects of sensitivity on physicochemical treatment, typing of nucleic acid, hemagglutinating activity, rep...
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https://www.riss.kr/link?id=A19592282
1995
Korean
510.000
학술저널
1-7(7쪽)
0
상세조회0
다운로드다국어 초록 (Multilingual Abstract)
The Korean isolates of porcine reproductive and respiratory syndrom(PRRS) virus, KPRRSV-1 and KPRRSV-2, were characterized on the aspects of sensitivity on physicochemical treatment, typing of nucleic acid, hemagglutinating activity, rep...
The Korean isolates of porcine reproductive and respiratory syndrom(PRRS) virus, KPRRSV-1 and KPRRSV-2, were characterized on the aspects of sensitivity on physicochemical treatment, typing of nucleic acid, hemagglutinating activity, replication in cultured cells and morphology in electron microscopy.
The isolates were labile to ether or chloroform, acid(pH 3.0) or alkali(pH 8.0), and heating(56℃, 30min). The viruses showed no haemagglutinating activity, and inhibition of replication in the medium containing 5-bromo-2'-deoxyuridine. Virus replication was more active in porcine alveolar macrophage(PAM) as compared with that in MARC-145 cell line, showing the maximum titer of 10^4.6TCID_50/㎖ at 5 days post-exposure(pe). Cytopathic effects(CPE) were observed more evidently in the PAM cells than in MARC-145 cell line. In electron microscopy, observation for the isolates showed oval or spherical types of virions, surrounded by a thin membrane-like envelope. The virions were from 45 to 86nm in diameter containing the cores of 35∼50nm in size
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