Anticancer Activity of Cobra Venom Polypeptide, Cytotoxin-II, against Human Breast Adenocarcinoma Cell Line (MCF-7) via the Induction of Apoptosis

다국어 초록 (Multilingual Abstract)

Purpose: Breast cancer is a significant health problem worldwide,accounting for a quarter of all cancer diagnoses in women.
Current strategies for breast cancer treatment are not fully effective,and there is substantial interest in the identification of novelanticancer agents especially from natural products includingtoxins. Cytotoxins are polypeptides found in the venom of cobrasand have various physiological effects. In the present study,the anticancer potential of cytotoxin-II against the human breastadenocarcinoma cell line (MCF-7) was investigated. Methods:The cytotoxic effects of cytotoxin-II were determined by morphologicalanalysis and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide assay. The mode and mechanism of celldeath were investigated via acridine orange/ethidium bromide(AO/EtBr) double staining, flow cytometric analysis of cell death,detection of mitochondrial membrane potential, measurement ofintracellular reactive oxygen species (ROS), annexin V/propidiumiodide staining, and caspase-9 activity assays. Results: The halfmaximal inhibitory concentration (IC50) of cytotoxin-II in MCF-7cells was 4.18±1.23 μg/mL, while the value for cisplatin was approximately28.02±1.87 μg/mL. Morphological analysis and AO/EtBr double staining showed typical manifestations of apoptoticcell death (in doses lower than 8 μg/mL). Dose- and time-dependentROS generation, loss of mitochondrial membrane potential,caspase-9 activation, and cell cycle arrest were observed in theirrespective tests. Conclusion: In conclusion, cytotoxin-II has potentanticancer effects in the MCF-7 cell line, which are inducedvia the intrinsic pathways of apoptosis. Based on these findings,cytotoxin-II is a suitable choice for breast cancer treatment.

참고문헌 (Reference)

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