감초 추출물이 glutathione S-transferase의 유도 활성에 미치는 영향 = Induction of Glutathione S-transferase Activity by the Extracts of Glycyrrhiza uralensis Fischer

다국어 초록 (Multilingual Abstract)

In the present study, we investigated the glutathione S-transferase (GST) induction of medicinal plants using a cultured PC12 cells. The methanol extracts of Dendrobium nobile Lindley, Schizonepeta tenuifolia Briquet, Glycyrrhiza uralensis Fischer, Paenoia lactiflora Pall were tested. As the result, exposure of PC12 cells to G. uralensis resulted in the significant induction of GST. On the continuous experiment, G. uralensis was extracted with methanol, ethanol, and acetone. Among these extracts, methanol extracts showed the highest GST induction. The methanol extracts were further fractionated with hexane, diethyl ether, ethyl acetate, and water layer according to the degree of polarity. The diethyl ether layer showed the highest exhibited GST induction on PC12 cells. Based on these results suggest that the extracts of G. uralensis can be applicable for the development of a new antioxidant agent.

국문 초록 (Abstract)

를 가지고 있는 석곡, 형개, 감초 및 작약 추출물을 대상으로GST 유도 활성 효과를 실험하였다. 그 결과, 감초 추출물이 가장 높은 GST 유도 활성을 나타내었다. 감초를 methanol, ethanol, acetone으로 분획 추출한 뒤 GST 유도 활성을 살펴본결과, 감초의 methanol 추출물을 50 μg/ml 농도로 처리 시 대조구에 비해 2.23배로 가장 높은 GST 유도활성이 나타났다. 그리고 감초의 각 추출물들은 처리 농도에서 세포의 형태학적인변화를 유도하지 않았으며, 이는 LDH release assay 결과를 통해서도 매우 낮은 세포 독성을 확인할 수 있었다. 감초의 GST 유도 활성 물질들의 용매특성을 알아보기 위하여 methanol 추출물을 극성도에 따라 용매 분획하여 GST 유도 활성을 확인한 결과, diethyl ether층 분획물에서 가장 높은 GST 유도 활성을 나타냄을 확인하였다.

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