<P>Infection with dengue virus (DENV) is a serious health issue that causes severe dengue fever and occasionally lethal complications, such as dengue hemorrhagic fever. Therefore, rapid and sensitive DENV detection is important to reduce morbidi...
http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
https://www.riss.kr/link?id=A107468503
2018
-
SCI,SCIE,SCOPUS
학술저널
12607-12614(8쪽)
0
상세조회0
다운로드다국어 초록 (Multilingual Abstract)
<P>Infection with dengue virus (DENV) is a serious health issue that causes severe dengue fever and occasionally lethal complications, such as dengue hemorrhagic fever. Therefore, rapid and sensitive DENV detection is important to reduce morbidi...
<P>Infection with dengue virus (DENV) is a serious health issue that causes severe dengue fever and occasionally lethal complications, such as dengue hemorrhagic fever. Therefore, rapid and sensitive DENV detection is important to reduce morbidity and mortality. Here, we screened dengue NS1-specific affibodies and developed highly stable anti-NS1 affibody-functionalized AuNPs to improve the detection sensitivity of the NS1 antigen in ELISA. First, we screened NS1 antigen-specific affibody molecules (ZNS112, ZNS116, and ZNS146) from an affibody phage library. The affibodies were then expressed and purified from <I>Escherichia coli.</I> Among them, the ZNS112 affibody showed the highest equilibrium binding constant (<I>K</I>d) of 1 μM and was sufficiently stable at high temperatures. This affibody was functionalized on AuNPs measuring 20 nm in diameter, which were used as carriers to achieve amplification of the signal in ELISA. The developed anti-NS1 affibody-functionalized AuNPs ((ZNS112)2-AuNP) showed good properties, such as easy synthesis, a high number of affibody conjugations on the AuNPs, and excellent stability under harsh conditions with high salt concentrations and temperature. In addition, this nanoparticle-based enhanced ELISA resulted in a 14.2-fold signal amplification performance for dengue NS1 detection in comparison with the affibody-based ELISA. This novel and sensitive method using (ZNS112)2-AuNPs may have applications in the detection of DENV in infected patients at an early stage and for the detection of other pathogens in clinical diagnostics.</P>