Effects of Exercise on the Expression of 5-HT and TPH in the Raphe Nucles of Autism-treated Rats

국문 초록 (Abstract)

임신중 Valproic Acid 투여는 태어난 새씨 쥐에서 자폐증과 같은 행동을 유발한다. 세로토닌은 뇌의 신경 활성을 나타내는 요인으로 뇌의 발달에 중요한 역할을 하는 화학물질이다. 운동은 뇌에서 신경 가소성 및 활성을 증진시키는 요소로 알려져 있다. 본 연구에서는 임신 중 자폐증에 노출된 쥐로부터 태어난 새끼쥐의 봉선핵에서 세로토닌 합성 및 세로토닌 합성을 조절하는 효소인 5-HT 및 TPH의 과-발현증에 수영운동이 미치는 효과를 살펴보았다. 실험결과, 어미 의 자폐증 노출은 새끼 쥐의 봉선핵에서 세로토닌 합성 및 TPH 과발현증으로 불안과 철회행동과 같은 반응을 일으키는 것으로 나타나며, 이러한 반응은 수영 운동을 통해 이와 같은 영향이 억제가 되는 것으로 나타났다. 이와 같은 결과를 바탕으로 수영 운동은 임신 중 자폐증에 노출된 모체로부터 태어난 새끼 쥐들의 5-HT 및 TPH 과-발현증을 감소시킴으 로써 뇌의 기능적인 영향에 유용한 전략적 방안이 될 수 있을 것으로 보여 진다.

다국어 초록 (Multilingual Abstract)

Using rats exposed prenatally to valproic acid (VPA) as an animal model, we measured expression of 5-HT and TPH in samples collected from the dorsal raphe using the immunohistochemistry. The basal 5-HT and TPH expression level in raphe was significantly higher in VPA-exposed rats relative to controls. Since the over-expression of 5-HT and TPH system is known to be sensitive to physical and psychological stressors, we measured expression 5-HT and TPH levels in raphe nucles after chronic swimming exercise. There were further gradual decreased in hyperserotonemia expression 5-HT and TPH levels during the swimming exercise in the VPA-exposed rats and the level of serotonine decreased in the control rats. The expression 5-HT and TPH revealed a significant decrease in exercise with autism-treated rats. These results suggest that this rodent model of autism exhibits a high expression 5-HT and TPH, which is inhibited by swim exercise. This abnormality may be responsible for anxiety and withdrawal behavior observed in autism. Based on these present results, swimming exercise may be a useful strategy to decrease the hyperserotoninemia such as overexpression 5-HT and TPH of the pups born from mothers who have exposed VPA-treated rats during pregnancy.

목차 (Table of Contents)

• 요약
• Abstract
• Ⅰ. Introduction
• Ⅱ. Materials and Methods
• 1. Animals and Treatments
• 2. Exercise protocols
• 3. Tissue Preparation
• 4. Immunohistochemistry for TPH and 5-HT
• 5. Data Analysis
• Ⅲ. Results
• 1. Effect of postnatal swimming exercise on the TPH synthesis in the dorsal raphe of rat pups born from the autism-treated maternal rats
• 2. Effect of postnatal swimming exercise on the 5-HT synthesis in the dorsal raphe of rat pups born from the autism-treated maternal rats
• Ⅳ. Discussion
• References