Berberine Sulphate 를 이용한 형광현미경기법에 의한 수목마이코플라스마검정 = Detection of Mycoplasma - like Organisms in Some Trees by Fluorescence Microscopy with Berberine Sulphate

다국어 초록 (Multilingual Abstract)

The efficacy of berberine sulphate, a fluorochrome having binding properties with both DNA and RICA, was investigated for the detection of mycoplasma-like organisms(MLOs) in jujube(Zizyplzus jujuba), paulownia (Paulounia tomentosa), mulberry(Morus alba) trees and periwinkle (Catharantlaus roseus) plant. When examined under fluorescence microscope, berberine sulphate-stained sections of diseased samples showed distinct MLO-specific fluorescent particles in the phloem area, while such fluorescence was absent in the healthy ones. This staining technique was proved to be a very accurate method for the diagnosis of MLO infections in woody and herbaceous plants. Furthermore, the cheap and easy procedure could be used to test a, great number of samples on MLO infections with reliability and rapidity.