Brazzein protein production in transgenic carrot cells using air-lift bioreactor culture

다국어 초록 (Multilingual Abstract)

Recombinant-protein production in plants is a more effective system than the mammalian cell system because it can be more easily scaled up, has a low production cost, and is animal pathogen free. In this study, we established a brazzein production system using an air-lift bioreactor and transgenic carrot cells. The system included the introduction of the brazzein gene by Agrobacterium-mediated transformation. First, transgenic carrot callus (TC12) was cultured in a liquid medium. The brazzein content was analyzed during entire culture period. Next, the bioreactor type, volume, and culture period were compared to establish a bioreactor culture system. In addition, abscisic acid (ABA) was treated as an elicitor to increase brazzein bio- synthesis. During the suspension culture, cell proliferation increased rapidly beginning at day 12. At that time, total soluble protein (TSP) and brazzein were 9.0 mg·g FW−1 and 1.2 mg·g FW−1, respectively. Brazzein content was 0.5 µg·g FW−1 (TSP 5.3 mg·g FW−1) in the balloon bioreactor. Cone and column bioreactors produced 1.1 µg·g FW−1 (TSP 5.7 mg·g FW−1) and 1.2 µg·g FW−1 (TSP 7.8 mg·g FW−1), respectively. The cone bioreactor was the most suitable for brazzein cell proliferation (6.1 g brazzein per bioreactor). After 8 h of 50 μM ABA treatment, the brazzein content had increased by 3.3-fold compared to that in the non-treated bioreactor. Overall, the results show that brazzein can be produced from carrot cell culture and an air-lift bioreactor system can be used for industrial mass production.

참고문헌 (Reference)

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