Establishment of bioreactor cultures for the production of chlorogenic acid and ferulic acid from adventitious roots by optimization of culture conditions in Angelica acutiloba (Siebold & Zucc.) Kitag|RISS 상세보기

다국어 초록 (Multilingual Abstract)

Adventitious roots were induced from root explants of Angelica acutilobaand suspension cultures were established in shake flasks. The effect of different concentrations of indole acetic acid (IBA, 0–38.4 µM), the strength of the Murashige and Skoog (MS) medium (0.5–2 ×), the concentration of sucrose (10–70 g·L−1), inoculum density (2.5–10 g·L−1) were determined for accumulation biomass. The optimized conditions for adventitious root biomass accumulation were found to be full-strength MS medium supplemented with 29.5 µM IBA, 30 g·L−1 of sucrose, and 5 g·L−1 of inoculum density. With optimized con- dition, adventitious root suspension cultures were established in 3 L capacity airlift bioreactors using 2 L of MS medium. Chlorogenic acid and ferulic acid were increased 4.9 (117.7 mg g·g−1 DW) and 1.9 times (47.2 mg g·g−1 DW), respectively, compared to control (0 week) after 4 weeks of culture in the optimized conditions. The results suggested that the harvested adventitious root biomass could able accumulate higher concentrations of chlorogenic acid and ferulic acid which could be used industrial purpose as a biomaterials.

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참고문헌 (Reference)

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