항히스타민/비만세포 안정화 복합제재의 결막세포에 대한 효능 비교

다국어 초록 (Multilingual Abstract)

Purpose: To investigate the biologic effects of topical anti-allergic agents with H1-receptor antagonism and inhibition of histamine release from mast cells in the cultured conjunctival cells of patients with vernal keratoconjunctivitis in vitro. Methods: Conjunctival cells of vernal keratoconjunctivitis were exposed to the anti-allergic agents SCD-P101 (Fexofenadine, Samchundang, Korea), PatanolⓇ (Alcon, USA), ZaditenⓇ (Novartis, USA), and AzelanⓇ (Taejoon, Korea). Efficacy of the topical antihistamine/mast cell stabilizers was evaluated using the MTT assay, measuring the concentration of procollagen and inflammatory cytokines. Cell damage was determined using the lactate dehydrogenase (LDH) assay with dilution rates of 10, 20, and 30% and compared with the balanced salt solution-treated group. Cellular morphologic results were examined by inverted light microscopy and transmission electromicroscopy. Results: Metabolic activity of conjunctival cells decreased at higher concentrations and longer exposure durations, except for the SCD-P101 agent. The procollagen, laminin, IL-6 and IL-8 titers tended to be lower than that of the control in the eyes exposed to all the anti-allergic drugs tested in this study, but the concentration of TNF-β was similar to that of the control group. ZaditenⓇ and AzelanⓇ tended to show a greater LDH titer and edema, as well as cytoplasmic and nuclear degeneration of the conjunctival cells than did SCD-P101 or PatanolⓇ. Conclusions: Cellular metabolic activity was the highest in the new anti-allergic agent SCD-P101. SCD-P101 and PatanolⓇ caused marginally less damage to cultured conjunctival cells than did ZaditenⓇ and AzelanⓇ. J Korean Ophthalmol Soc 2010;51(3):406-417

참고문헌 (Reference)

1 Bielory L, "Therapeutic targets in allergic eye disease" 22 : 25-28, 2001

2 Lee JS, "The effects of artificial tear formulation and anti-inflammatory agents on the cultured keratocyted of rabbit" 39 : 42-51, 1998

3 Freshney RI, "The culture environment: Substrate, gas phase, medium, and temparature, 2nd ed" Wiley-Liss 70-71, 1987

4 Sarac OI, "Tear eotaxin levels in giant papillary conjunctivitis associated with ocular prosthesis" 11 : 223-230, 2003

5 Mosmann TJ, "Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxic assays" 65 : 55-63, 1983

6 Spangler DL, "Randomized, double-masked comparison of olopatadine ophthalmic solution, mometasone furoate monohydrate nasal spray, and fexofenadine hydrochloride tablets using the conjunctival and nasal allergen challenge models" 25 : 2245-2267, 2003

7 Burstein NL, "Preservative cytotoxic threshold for benzalkonium chloride and chlorhexidine digluconate in cat and rabbit corneas" 19 : 308-313, 1980

8 Park YS, "Physiology of body fluid. In Physiology, 5th ed" Sin-Kwang publishing & printing 5-, 2000

9 Schultz BL, "Pharmacology of ocular allergy" 6 : 383-389, 2006

10 Abelson MB, "Ocular allergic disease: Mechanisms, disease sub-types, treat" 1 : 127-149, 2003

11 Leonardi A, "Multiple cytokines in human tear specimens in seasonal and chronic allergic eye disease and in conjunctival fibroblast cultures" 36 : 777-784, 2006

12 Frossard N, "Inhibition of allergen-induced wheal and flare reactions by levocetirizine and desloratadine" 65 : 172-179, 2007

13 Green K, "Influence of various agents on corneal permeability" 72 : 897-905, 1971

14 Holgate ST, "Improving the management of atopic disease" 90 : 826-831, 2005

15 Foster CS, "Immunologic disorders of the conjunctiva, cornea, and sclera In Principles and Practice of Ophthalmology" WB Saunders Company 65-, 1994

16 Luo L, "Hyperosmolar saline is a proinflammatory stress on the mouse ocular surface" 31 : 186-193, 2005

17 Weeke ER, "Epidemiology of hay fever and perennial allergic rhinitis" 21 : 1-20, 1987

18 Wee RW, "Effect of anrtificial tears on cultured keratocytes in vitro" 14 : 273-279, 1995

19 Lee JS, "Cytotoxicity of banzalkonium chlorid on the corneal epithelial cell of rabbit" 39 : 1326-1333, 1998

20 Cha SH, "Corneal epithelial cellular dysfunction from banzalkonium chloride (BAC) in vitro" 32 : 180-184, 2004

21 Lee JS, "Comparison of the conjunctival toxicity of topical ocular antiallergic agents" 24 : 557-562, 2008

22 Lee SJ, "Body fluid and acid-base balance. In Physiology" Eui-hak publishing & printing 20-, 2002

23 McGill JI, "A review of the use of olopatadine in allergic conjunctivitis" 25 : 171-179, 2004