DNCB로 유발된 생쥐의 아토피 피부염에 대한 지실(枳實)의 효능 = Effects of Aurantii Immaturus Fructus (AI) on Atopic Dermatitis (AD) Induced by DNCB in Mice

다국어 초록 (Multilingual Abstract)

Objectives The purpose of this study was to investigate the effects of AI on AD induced by DNCB in mice. AI has antiallergic property that is useful in treating allergy-related-diseases, such as asthma, anaphylactic shock, acute bronchitis and skin diseases, skin pruritus from gastrointestinal diseases. However, AI has not been studied intensively yet regarding anti-inflammatory effect on AD. Therefore, this study was conducted on 2,4-dinitrochlorobezene (DNCB)-induced mice to investigate effects of AI in AD. Methods In the experiment, we divided mice into four groups: a normal group (NOR), a control group (CON), an AI spread group (AI spread), and an AI spread and feeding group (AI spread & feeding). Then examined the changes in the body weight, weights of spleen and ear, thickness of dorsum skin and ear skin, clinical aspects on dorsum skin, historical assessments, proliferation of splenocytes in vitro and in vivo, and cytokine (TNF-${\alpha}$, IL-10). Results From the experiment, the ear weight of AI spread & feeding group was significantly dropped and the ear thickness of both AI spread and AI spread & feeding were decreased significantly. Dorsum skin thickness was also decreased significantly in both AI spread and AI spread & feeding group. Also, AI treatment improved the symptoms of AD, such as coloration, erythema and desquamation and had a better effect on AI spread & feeding group. In histopathological observation, thickened epidermis, hyperkeratosis, pigmentation, hypergranulosis, parakeratosis were diminished as well in both AI spread and AI spread & feeding group. In vitro, we could observe when AI was increased as proliferation rate of splenocytes were increased, too. Conclusions In conclusion, these data suggest that AI can decrease symptoms of AD and show AI can be useful herbal therapy for AD.

참고문헌 (Reference)

1 손은화, "홍삼의 생체 내 사이토카인 분비에 대한 면역조절효과" 한국자원식물학회 25 (25): 578-585, 2012

2 조민서, "한방 병원 소아과를 내원한 소아 아토피 피부염 환자 실태에 관한 조사" 대한한방소아과학회 25 (25): 8-14, 2011

3 박경숙, "인간의 단핵구와 비만세포에서 다양한 아토피 유발물질이 사이토카인 유전자의 발현에 미치는 영향" 대한한방안이비인후피부과학회 23 (23): 41-56, 2010

4 박용민, "아토피피부염 병태생리에 대한 최신 지견" 대한 소아알레르기 호흡기학회 16 (16): 189-196, 2006

5 홍정효, "아토피의 치료에 관한 양방과 한방, 민간요법과 자연치유법에 관한 고찰" 한국생태유아교육학회 7 (7): 65-85, 2008

6 홍원규, "소아 아토피 피부염에서 발아현미 상황버섯의 임상적 치료효과" 대한본초학회 23 (23): 103-108, 2008

7 장지혜, "소아 아토피 피부염에 관한 임상적 고찰" 대한한방소아과학회 19 (19): 69-84, 2005

8 안종현, "선퇴가 인간의 THP-1 단핵구에서 사이토카인 유전자 발현에 미치는 영향" 대한한방안이비인후피부과학회 23 (23): 94-110, 2010

9 류혜숙, "생강 추출물 투여에 의한 마우스 비장세포 및 대식세포 활성 효과" 한국영양학회 37 (37): 780-785, 2004

10 심태경, "별갑이 아토피 피부염에서의 알러지성 염증 반응에 미치는 영향" 한의학연구소 20 (20): 69-83, 2011

11 박경철, "급성췌장염 마우스 모델에서 지실과 지각 추출물의 보호효과" 대한본초학회 27 (27): 9-14, 2012

12 안상현, "금은화의 NF-κB 활성 억제를 통한 iNOS 조절이 NC/Nga 생쥐의 아토피 피부염에 미치는 영향" 한의병리학회 24 (24): 278-283, 2010

13 서부일, "고지방 식이로 유도된 비만 흰쥐에 茵蔯, 枳實, 山？가 미치는 영향" 대한본초학회 21 (21): 55-67, 2006

14 정인조, "개에서 실험적으로 유발한 비장종대의 방사선학적 평가" 한국임상수의학회 23 (23): 263-266, 2006

15 정우영, "감잎(柿葉) 추출물의 채취시기에 따른 항산화 활성 변화 및 아토피성 피부염 개선효과" 대한본초학회 27 (27): 41-49, 2012

16 Wang BD, "Yookgwajoonseung" Seongbosa 85-87, 1982

17 Chang IM, "Treatise on asian herbal medicines" Seoul National Univ Press Inc 176-177, 2003

18 Song BK, "The study on the antiallergic action of Poncirus trifoliata" 21 (21): 156-161, 2000

19 Huang KC, "The pharmacology of Chinese Herbs" CRS Press 108-, 1994

20 Aeom YD, "The comparative study of Fructus Immaturus Ponciri and Fructus Ponciri effect on allergic reaction" 22 (22): 10-21, 2001

21 Jo HC, "Sungjechonglok" Shinmoonnong 1439-1440, 1978

22 Chun YT, "Screening of antiallergic effect in traditional medicinal drugs and active constituents of Aurantii Fructus Immaturus" 43 : 314-323, 1989

23 Baek YH, "Salviae miltiorrhizae root inhibits atopic dermatitis in the DNCB-induced atopic dermatitis NC/Nga mice" Won Kwang Uni 2005

24 Park MC, "Review literatures of East-West medical in atopic dermatitis" 15 (15): 226-252, 2002

25 Kim HJ, "Relation between atopic dermatitis and residential environment" Yonsei Uni 2005

26 Mizel SB, "Regulation of lymphocyte-activating factor(LAF)production and secretion in P388D1 cells ; identification of high molecular weight precursors of LAF" 122 (122): 2173-2179, 1979

27 Ministry of Health and Welfare, "National health and nutrition examination survey"

28 Hwang GH, "Mechanism of inhibitory action of anaphylaxis by aqueous extract of Poncirus trifoliata" 18 (18): 316-325, 1997

29 Kim KB, "Kor Pediatrics Tx" Euhsungdang 751-752, 2010

30 So WB, "Jebyungwonwhoron" Jipmoonseogook 505-, 1965

31 Lee JW, "Inhibitory effect of immediate-type allergic reaction of Poncirus trifoliata L. by anal therapy" 13 (13): 116-128, 2000

32 Jo SY, "Hwalyousimseo" Daedongseogook 2-8, 1933

33 College of Korean Medicine co association Editorial Board, "Herbalism Tx" Younglimsa 395-396, 2004

34 Yang JM, "Genetics of atopic dermatitis. Update in dermatology for a dermatologist"

35 Rho SS, "Full-color Dermatology" IBC Design 499-, 2006

36 Lee JK, "Evaluation of immunosafety for skin sensitization induced by chemicals" 4 (4): 81-90, 2005

37 Kim JK, "Effect of Scrophularia Buergeriana extract on the degranulation of mast cells and ear swelling induced by dinitrofluorobenzene in mice" 35 (35): 183-191, 2012

38 Huh J, "Dongeubogam" Dongeubogam 730-731, 2006

39 Kim CM, "Dictionary of Chinese medicine" Jeongdam 3969-3976, 1997

40 Society of Dermatology, Textbook Compilation Committee, "Dermatology 5th Edition" Yeoumoongak 95-, 2008

41 De Blaauw I, "De novo glutamine synthesis induced by corticosteroids in vivo in rats is secondary to weight loss" 23 (23): 1035-1042, 2004

42 Jung JY, "Common skin diseases in Korea" Emdi world 37-48, 2009

43 Kim KH, "Clinical study for the efficacy of external prepartion containing Sophorae Radix, Aurantiii Immaturus Fructus, Schizonepetae Spica, Forsythiae Fructus and Portulacea Herba extract on atopic dermatitis" Daejeon Univ 2013

44 Kim JP, "Chinese Dermatology magazine"

45 Leung DYM, "Atopic dermatitis : new insights and opportunities for therapeutic intervention" 105 : 860-876, 2000

46 Shin TY, "Allergies and herbal medicine" Shinilsangsa 3-, 2001

47 Saint-Mezard P, "Allergic contact dermatitis" 14 (14): 284-295, 2004

48 Kim JW, "Allergic and immunological point of view of atopic dermatitis" 41 (41): 687-689, 2003

49 Kim MJ, "A literature study of atopic dermatitis for children" 14 (14): 169-183, 2000

50 Kim JM, "A clinical study on atopic dermatitis" 15 (15): 220-227, 2002

51 Wysocki LJ, "'Planning' for lymphocytes ;a method for cell selection" 75 (75): 2844-2848, 1978