간 질환시 동양주위세포의 알파-평활근 액틴 표출에 대한 면역조직 화학적 연구 = A Study on the Expression of α-Smooth Muscle Actin in Perisinusoidal Cells of Liver Diseases

다국어 초록 (Multilingual Abstract)

The various liver diseases result in fibrosis, which is irreversible and pregressively replace normal hepatic parenchyma finally bringing out hepatic failure. Though It has not been elucidated which cells of the liver parenchyma are origin of hepatic fibrosis, perisinusoidal cells(PSCs) are thought to play a pivotal role in the pathogenesis of fibrocontractive changes in the late stage of various liver diseases. By light and electron microscopic immunohistochemistry, α-smooth muscle actin(α-SMA) is known to be the cytoskeletal marker of the fibrocontractibe cells either in granulation tissue of wound healing or in hepatic fibrosis. Several lines of evidence showed that the PSCs in fibrocontracted liver expressed the α-SMA strongly
This study was done to evaluate the expression of α-SMA in various liver diseases
The results were as follows;
1. Normal fetal liver showed mild α-SMA expression in periportal zone. The PSCs in intermediate zone and pervenulargions showed only negligible stain for α-SMA.
2. The chronic active hepatits revealed moderate α-SMA expression in periportal zone, especially in parenchymal necrotic region. The PSCs in intermediate zone and peribenular region showed mild α-SMA expression.
3. The chronic persistent hepatitis showed only mild α-SMA expression in periportal zone of mild inflammatory reaction. The intermediate zone and perivenular region showed negligible immunostain.
4. Alcoholic hepatitis showed strong α-SMA expression in perivenular region and the PSCs in intermediate zone also showed moderate immunoractibity.
5. The cirrhotic region after alcoholic hepatitis and chronic active hepatitis showed strong immunoreactibity ofr α-SMA in fibrous septa.
The above result suggests that the mesenchymal cells expressing α-SMA as PSCs be responsible for the fibrosis of various liver diseases. As the expression of α-SMA precede the fibrocontraction of the liver, immunohistochemistry for α-SMA in various liver diseases would be necessory to predict the prognosis of the liver diseases.