cox1 분자마커를 이용한 한국산 패류 천공성 다모류 Polydora haswelli (Polychaeta, Spionidae) 유전자 다양성 발굴 = Genetic Diversity of Polydora haswelli (Polychaeta, Spionidae) in Korean Shellfish using cox1 Marker

다국어 초록 (Multilingual Abstract)

Harmful shell-boring species of the genus Polydora (Polychaeta: Spionidae) were frequently reported from commercially important mollusk species in Korea, Japan and China. The traditional approach based on the morphological characteristics showed limitations for species discrimination among shell-boring species. Therefore, DNA barcoding was adopted to identify Polydora species using molecular markers. Two Polydora species (P. haswelli and P. hoplura) in abalone shells were reported from our previous molecular phylogenetic study. In this study, we additionally reported the presence of shell-boring Polydora haswelli in commercially sold shellfish. The taxon-specific cox1 marker used in this study successfully allowed the isolation of P. haswelli from cockle Scapharca subcrenata, mussel Mytilus galloprovincialis, oyster Crassostrea gigas and scallop Argopecten irradians. Polydora hoplura was not found in these shellfish species. The genetic variations were found on the intraspecific level of P. haswelli and the same genotype was also detected in different shellfish species. This result can provide information on a new host and accurate parasitic Polydora species. Moreover, this report can be used as the biodiversity data of Polydora species on the invasion and transition of harmful Polydora species in mollusk aquaculture farms.

참고문헌 (Reference)

1 원경미, "우리나라 전복 양식장의 패각 천공성 다모류 감염현황" 한국어병학회 26 (26): 139-148, 2013

2 이덕찬, "양식전복 패각에서 분리한 다모류의 유전학적 분류" 한국패류학회 36 (36): 157-165, 2020

3 Statistics Korea, "The result of 2020 fishery production trend survey (provisional)"

4 Lee JW, "Systematic study of polychaetes(Annelida)from offshore waters of Geojedo Island, Korea" 14 : 243-255, 1998

5 W. Sato-Okoshi, "Polydorid species (Polychaeta: Spionidae) associated with commercially important mollusk shells in Korean waters" Elsevier BV 350-353 : 82-90, 2012

6 CA Simon, "Polydorid polychaetes on farmed molluscs: distribution, spread and factors contributing to their success" Inter-Research Science Center 7 (7): 147-166, 2015

7 National Institute of Biological Resources, "National list of species of Korea. Invertebrate-VII" Ministry of Environment and National Institute of Biology Resources 107-, 2015

8 W Teramoto, "Morphology, 18S rRNA gene sequence and life history of a new Polydora species (Polychaeta: Spionidae) from northeastern Japan" Inter-Research Science Center 18 (18): 31-45, 2013

9 W. Sato-Okoshi, "Morphological and molecular sequence analysis of the harmful shell boring species of Polydora (Polychaeta: Spionidae) from Japan and Australia" Elsevier BV 368-369 : 40-47, 2012

10 Lingtong Ye, "Morphological and Molecular Characterization of Polydora websteri (Annelida: Spionidae), with Remarks on Relationship of Adult Worms and Larvae using Mitochondrial COI Gene as a Molecular Marker" ResearchersLinks Ltd 49 (49): 699-710, 2017

11 Lee-Gavin Williams, "Molecular identification of polydorid polychaetes (Annelida: Spionidae): is there a quick way to identify pest and alien species?" Informa UK Limited 52 (52): 105-117, 2017

12 Soon Jeong Lee, "Molecular Detection for Two Abalone Shell-Boring Species Polydora haswelli and P. hoplura (Polychaeta, Spionidae) from Korea using 18S rDNA and cox1 Markers" 한국해양과학기술원 55 (55): 459-464, 2020

13 Koichiro Tamura, "MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0" Oxford University Press (OUP) 30 (30): 2725-2729, 2013

14 National Institute of Biological Resources, "Korea biodiversity"

15 LN Rice, "Genetic homogeneity among geographically distant populations of the blister worm Polydora websteri" Inter-Research Science Center 10 : 437-446, 2018

16 Vasily I. Radashevsky, "First report of the polychaete Polydora hoplura (Annelida: Spionidae) from North and South America and Asian Pacific" Springer Science and Business Media LLC 47 (47): 859-868, 2017

17 L. Williams, "Dispersal and genetic structure of Boccardia polybranchia and Polydora hoplura (Annelida: Spionidae) in South Africa and their implications for aquaculture" Elsevier BV 465 : 235-244, 2016

18 Mark M. Bouwmeester, "Collateral diseases: Aquaculture impacts on wildlife infections" Wiley 58 (58): 453-464, 2021

19 Paul D. N. Hebert, "Biological identifications through DNA barcodes" The Royal Society 270 (270): 313-321, 2003