사료 내 Bacillus 균주의 첨가가 넙치(Paralichthys olivaceus) 치어의 성장, 사료효율, 선천성면역 및 연쇄구균 질병저항성에 미치는 영향 = Effects of Dietary Supplementation with Bacillus sp. on Growth Performance, Feed Utilization, Innate Immunity and Disease Resistance against Streptococcus iniae in Olive flounder Paralichthys olivaceus

다국어 초록 (Multilingual Abstract)

We investigated the effect of dietary supplementation with probiotics on growth performance, feed utilization, innate immunity and disease resistance against Streptococcus iniae in juvenile olive flounder Paralichthys olivaceus. Four experimental diets were made by supplementation with three Bacillus sp. probiotics (B. subtilis, B. pumilus and B. licheniformis)into a basal diet (control) at 0.5% each. Three-hundred fish (average BW, 20 g) were randomly distributed (25 per tank) into twelve 150 L capacity polyvinyl circular tanks with three replicates per dietary treatment. Triplicate groups of fish were fed the experimental diets to apparent satiation (twice per day, 09:00 and 17:00 h) for nine weeks.
After the feeding trial, a challenge test was directly conducted against S. iniae. The results indicated that dietary supplementation with the probiotic, Bacillus sp, seemed to improve the growth performance of olive flounder. Hematological parameters and innate immunity as determined by nitroblue tetrazolium, myeloperoxidase or superoxide dismutase activities were not significantly affected by the probiotic supplementation. The result from the challenge test suggested that dietary supplementation with probiotics Bacillus sp. can improve the disease resistance of olive flounder against S.
iniae.

참고문헌 (Reference)

1 김성삼, "해조류 (톳, 감태) 혼합물의 사료 내 첨가가 넙치의 선천성 면역과 질병저항성에 미치는 영향" 한국수산과학회 42 (42): 614-620, 2009

2 정창화, "치어기 넙치 사료내 생균제 첨가효과" 한국수산과학회 39 (39): 460-465, 2006

3 이은영, "축산 환경개선제로 생산·유통되는 생균제의 문제점 및 검증방안" 한국미생물·생명공학회 36 (36): 87-95, 2008

4 김성삼, "저수온기 넙치 치어에 있어서 발효마늘분말의 사료 내 첨가가 면역반응, 혈액성분 및 주요 어병세균에 대한 질병저항성에 미치는 영향" 한국동물자원과학회 52 (52): 337-346, 2010

5 박상현, "생균제 첨가사료가 조피볼락 Sebastes schlegeli의 성장 및혈액성분에 미치는 영향" 한국양식학회 21 (21): 1-6, 2008

6 Lara-Flores M, "Use of the bacteria Streptococcus faecium and Lactobacillus acidophilus, and the yeast Saccharomyces cervisiae as growth promoters in Niletilapia (Oreochromis niloticus)" 216 : 193-201, 2003

7 Robertson PAW, "Use of Carnobacterium sp. a probiotic for Atlantic salmon (Salmosalar L.) and rainbow trout (Onco-rhynchus mykiss, Walbaum)" 185 : 235-243, 2000

8 Vazquez-Juarez R, "The expression of potential colonization factors of yeasts isolated from fish during different growth conditions" 39 : 1135-1141, 1993

9 Ziaei-Nejad S, "The effect of Bacillus spp. bacteria used as probiotics on digestive enzyme activity, survival and growth in the Indian white shrimp Fenneropenaeus indicus" 252 : 516-524, 2006

10 Ziaei-Nejad S, "The effect of Bacillus spp. bacteria used as probiotics on digestive enzyme activity, survival and growth in the Indian white shrimp Fenneropenaeus indicus" 252 : 516-524, 2006

11 Vanbelle M., "The European perspective on the use of animal feed additives. In Biotechnology in the 7. Feed Industry" Nottingham University Press 375-, 1989

12 Aly SM, "Studies on Bacillus subtilis and Lactobacillus acidophilus, as potential probiotics, on the immune response and resistance of Tilapia (Oreochromis niloticus) to challenge infections" 25 : 128-136, 2008

13 Garrido LG, "Serum cholesterol and triglyceride levels in Scyliorhinus during sexual maturation" 36 : 499-509, 1990

14 Bakke-Mckellep AM, "Response to soy: T-celllike reactivity in the intenstine of Atlantic salmon, Salmosalar" 30 : 13-25, 2007

15 Mohanty BK, "Relationship between sequence-specific termination of DNA replication and transcription" 15 : 2530-2539, 1996

16 De-Schrijver, R, "Protein digestion in Juvenile turbot (Scophthalmus maximus) and effects of dietary administration of Vibrio proteolyticus" 186 : 107-116, 2000

17 Gidberg A, "Probiotic effect of lactic acid bacteria in the feed on the growth and survival of fry of Atlatic cod (Gadus morhua)" 352 : 270-285, 1997

18 Sun YZ, "Probiotic applications of two dominant gut Bacillus strains with antagonistic activity improved the growth performance and immune responses of grouper Epinephelus coioides" 29 : 803-809, 2010

19 Merrifield DL, "Probiotic application for rainbow trout (Oncorhynchus mykiss) II. Effects on growth performance, feed utilization, intestinal microbiota and related health criteria postantibiotic treatment" 10 : 1365-2095, 2009

20 AOAC, "Official Methods of Analysis, 16th ed" Association of Official Analytical Chemists 1298-, 1995

21 Gatesoupe FJ., "Lactic acid bacteria increase the resistance of turbot larvae, (Scophthalmus maximus) against pathogenic vibrio" 7 : 182-277, 1994

22 Zhang Q, "Interaction of dietary Bacillus subtilis and Fructooligosaccharideon the growth performance, non-specificimmunity of sea cucumber, Apostichopus japonicas" 29 : 204-211, 2010

23 Bogut I, "Influence of probiotic (Streptococcus faefium M74)on growth and content of intestinal micro florain carp(Cyprinuscarpio)" 43 : 231-235, 1998

24 Lauzon HI, "Impact of probiotic intervention on microbial load and performance of Atlantic cod (Gadus morhus L.) juveniles" 310 : 139-144, 2010

25 Bagheri T, "Growth, survival and gut microbial load of rainbow trout (Onchorhynchus mykiss) fry given diet supplemented with probiotic during the two months of first feeding" 8 : 43-48, 2008

26 Taoka Y, "Growth, stress tolerance and non-specific immune response of Japanese flounder Paralichthys olivaceus to probiotics in a closed recirculating system" 72 : 310-321, 2006

27 Fattman CL, "Extracellular superoxide dismutase in biology and medicine" 35 : 236-256, 2003

28 Skrodenyte-Arbaciauskiene V., "Enzymeatic activity of intestinal bacteria in roach Rutilus rutilus" 73 : 964-966, 2007

29 Bairagi A, "Enzyme producing bacterial flora isolated from fish digestive tracts" 10 : 109-121, 2002

30 Raida MA, "Enhanced resistance of rainbow trout, Oncorhynchus mykiss, against Yersinia ruckeri challenge following oral administration of B. subtilis and B. licheniformis" 26 : 495-498, 2003

31 Rychen G, "Effects of three microbial probiotics on postprandial concentration differences of glucose, galactose and amino-nitrogen in the young pig" centre de Recherche en Nutrition Animale 107-111, 1994

32 Abraham TJ, "Effects of dietary supplementation of commercial human probiotic and antibiotic on the growth rate and content of intestinal microflora in ornamental fishes" 11 : 57-63, 2007

33 Kumari K, "Effects of cyclophosphamide on the immune system and disease resistance of Asian catfish Clarias batrachus" 19 : 307-316, 2005

34 Balcazar JL, "Effect of the addition of four potential probiotic strains on the survival of pacific white shrimp (Litopenaeus vannamei) following immersion challenge with Vibrio parahaemolyticus" 96 : 147-150, 2007

35 Yeo SK, "Effect of prebiotics on viability and growth characteristics of probiotics in soymilk" 90 : 267-275, 2009

36 Santos JA, "Effect of lactic starter cultureon the growth and protease activity of Aeromonas hydrophila" 80 : 13-18, 1996

37 Geng X, "Effect of dietary probiotic on the growth performance, non-specific immunity and disease resistance of cobia" 10 : 1365-2095, 2011

38 Picchietti S, "Effect of administration of probiotic strains on GALT of larval gilthead seabream: Immunohistochemical and ultrastructural studies" 22 : 57-67, 2007

39 Son VM, "Dietary administration of the probiotic, Lactobacillus plantarum, enhanced the growth, innate immune responses and disease resistance of the grouper Epinephelus coioides" 26 : 691-698, 2009

40 Kim DH, "Cytokine expression in leucocytes and gut cells of rainbow trout, Oncorhynchus mykiss Walbaum, induced by probiotic" 114 : 297-304, 2006

41 Kennedy T, "Copper-dependent inflammation and Nuclear Factor-kB Activation by Particulate Air Pollution" 19 : 366-378, 1998

42 Vaseeharan B, "Control of pathogenic Vibrio spp. by Bacillus subtilis BT23, a possible prbiotic treatment for black tiger shrimp Penaeus monodon" 36 : 83-87, 2003

43 Ghosh K, "Characterization of bacilli isolated from the gut of rohu, Labeo rohita fingerlings and its significance in digestion" 12 : 33-42, 2002

44 Cleveland J, "Bacteriocins: safe, natural antimicrobials for food preservation" 71 : 1-20, 2001

45 Klaenhammer TR, "Bacteriocin of lactic acid bacteria" 70 : 337-349, 1988

46 Ministry of Maritime Affairs and Fisheries, "Aquaculture Statistic from Ministry of Maritime Affairs and Fisheries"

47 Avella MA, "Application of multispecies of Bacillus in seabream larviculture" 305 : 12-19, 2010

48 Rodriguez JC, "Antibiotic resistance during therapy: mechanisms and means of control" 7 : 43-45, 2007

49 Palic D, "A rapid, direct assay to measure degranulation of primary granules in neutrophils from kidney of fathead minnow (Pimephales promelas Rafiesque, 1820)" 19 : 217-227, 2005

50 Folch J, "A Simple Method for the Isolation and purification of Total Lipides from Animal Tissues" 226 : 497-509, 1957