노화된 생쥐의 근육에서 규칙적인 운동의 효과: 세포자멸사와 근육형성조절인자의 변화 = Effect of regular exercise in aging mouse: study on apoptosis, myogenesis regulatory factors (MyoD & Myogenin)

다국어 초록 (Multilingual Abstract)

The present study was designed to examine the antiapoptotic effects and expression of MayD and Myogenin in muscle of aging mouse by regular exercise. HSP70, MyoD and Myogenin protein expression were measured by western blot and apoptisis was detected by TUNEL in muscle tissue. Swiss-Vancouver mice at age of 8 weeks (n=10; Young) and 12months old (n=20; Old) were divided into 3 groups as follows: (1) Old control group (n=10, O-Con), (2) Young control group (n=10, Y-Con) (3) Old+exercise group (n=10, O-Ex). The regular exercise was performed 5 days /week with duration of 40minutes for 8weeks. Results showed that the number of apoptotic cell in Y-Con and O-Ex were decreased (p<.001, p<.05) compared to O-Con group. Particularly, the HSP70 expression in Y-Con and O-Ex was markedly up-regulated as compared with O-Con group. MyoD and Myogenin expression in O-Ex were significantly increased than O-Con groups. These results suggest that regular exercise should inhibit apoptosis by increasing HSP70, MyoD and Myogenin protein expression in muscle of O-Ex. Consequently, regular exercise may be preventive effect from natural aging of muscles.

참고문헌 (Reference)

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