복분자 열매, 잎, 줄기 추출물의 콜레스테롤과 혈압 개선 효과

다국어 초록 (Multilingual Abstract)

We compared effects of fruit, leaf, and stem extracts from black raspberry on improvement of cholesterol and blood pressure in HepG2 and HUVEC cells, respectively. Cholesterol secretion was inhibited by water extracts of unripe fruit and stem, but not leaf of black raspberry in HepG2 cells. Also, water extracts of unripe fruit, leaf, and stem reduced HMG-CoA reductase activity. Furthermore, nitric oxide production and expression of angiotensin-converting enzyme (ACE) protein were regulated by extracts of fruit, leaf, and stem of black raspberry in HUVEC cells. Overall, the rank order according to the improving level of cholesterol and hypertension is as follows: stem > fruit > leaf. In addition, various polyphenol compounds displayed inhibitory effects of HMG-CoA reductase activity and ACE expression. Thus, these data suggested that leaf and stem as wells as fruit of black raspberry can be used as useful food resources for reduction of cholesterol and blood pressure.

참고문헌 (Reference)

1 곽지현, "자색고구마 추출물의 항산화 효과 및 신경세포 보호효과" 농업생명과학연구원 44 (44): 57-66, 2010

2 김관후, "인삼 1차 부산물의 생산량 및 기능성 성분 특성" 한국약용작물학회 19 (19): 313-318, 2011

3 이미경, "복분자 열매 추출물의 유용 생리활성 탐색" 한국약용작물학회 11 (11): 5-12, 2003

4 권지웅, "복분자 에탄올 추출물의 생리활성 탐색" 한국약용작물학회 19 (19): 325-333, 2011

5 신미경, "대나무(이대)잎 추출물이 지방 및 고콜레스테롤 식이 급여에 의한 흰쥐의 지방 대사에 미치는 효과" 한국식생활문화학회 17 (17): 30-36, 2002

6 이소연, "瓊玉膏의 성분 분석 및 추출물별 抗酸化 효능 비교" 대한본초학회 23 (23): 123-136, 2008

7 Horowits JR, "Vascular endothelial growth factor/vascular permeability factor produces nitric oxide-dependent hypotension. Evidence for a maintenance role in quiescent adult endothelium" 17 : 2793-2800, 1997

8 Atlas SA., "The reninangiotensin aldosterone system : Pathophysiological role and pharmacologic inhibition" 13 : 9-20, 2007

9 Xie JT, "The composition and effects of ginseng leaf" 4 : 1-8, 2004

10 Brown MS, "The SREBP pathway : Regulation of cholesterol metabolism by proteolysis of a membrane-bound transcription factor" 89 : 331-340, 1997

11 Joyce C, "Study of ABCA1 function in transgenic mice" 23 : 965-971, 2003

12 Mullen E, "Soy isoflavones affect sterol regulatory element binding proteins(SREBPs)and SREBP-regulated genes in HepG2 cells" 134 : 2942-2947, 2004

13 Brundert M, "Selective uptake of HDL cholesteryl esters and cholesterol efflux from mouse peritoneal macrophages independent of SR-BI" 47 : 2408-2421, 2006

14 Horton JD, "SREPBs : Activators of the complete program of cholesterol and fatty acid synthesis in the liver" 109 : 1125-1131, 2002

15 Krause BR, "Reverse cholesterol transport and future pharmacological approaches to the treatment of atherosclerosis" 2 : 375-381, 2001

16 Han J, "Regulation of peroxisome proliferator-activated receptorgamma-mediated gene expression. A new mechanism of action for high density lipoprotein" 277 : 23582-23586, 2002

17 Taniguchi T, "Regulation of cholesterol 7 α-hydroxylase gene expression in Hep-G2 cells" 269 : 10071-10078, 1994

18 Sarr M, "Red wine polyphenols prevent angiotensin II-induced hypertension and endothelial dysfunction in rats : Role of NADPH oxidase" 71 : 794-802, 2006

19 Pal S, "Red wine polyphenolics increase LDL receptor expression and activity and suppress the secretion of ApoB100 from human HepG2 cells" 133 : 700-706, 2003

20 Mosmann T., "Rapid colorimetric assay for cellular growth and survival : Application to proliferation and cytotoxicity assays" 65 : 55-63, 1983

21 Bradford MM., "Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding" 72 : 248-254, 1976

22 Mahley RW, "Plasma lipoproteins : Apoprotein structure and function" 25 : 1277-1294, 1984

23 유기석, "Phylogenic Relationships of Rubus Species Revealed by Randomly Amplified Polymorphic DNA Markers" 한국작물학회 11 (11): 39-44, 2008

24 Moon SJ., "Nutritional problems of Korean" 29 : 371-380, 1996

25 Thomsen LL, "Nitric oxide synthase activity in human breast cancer" 72 : 41-44, 1995

26 Busse R, "Mechanism of nitric oxide release from the vascular endothelium" 87 : 18-25, 1993

27 Mckenny JM., "Lipid management : tools for getting to the goal" 7 : 299-306, 2001

28 Yotsumoto H, "Inhibitory effect of Oren-Gedoku-to and its components on cholesteryl ester synthesis in cultured human hepatocyte HepG2 cells : Evidence from the cultured HepG2cells and in vitro assay of ACAT" 63 : 141-145, 1997

29 Lim S, "Increasing prevalence of metabolic syndrome in Korea : The Korean National Health and Nutrition Examination Survey for 1998-2007" 34 : 1323-1328, 2011

30 Singh DK, "Green and black tea extracts inhibit HMG-CoA reductase and activate AMP kinase to decrease cholesterol synthesis in hepatoma cells" 20 : 816-822, 2009

31 Chang CC, "Estimation of total flavonoid content in propolis by two complementary colorimetric methods" 10 : 178-182, 2002

32 Godse S, "Effect of myricetin on blood pressure and metabolic alterations in fructose hypertensive rats" 48 : 494-498, 2010

33 National Statistical Office, "Death statistics in 2012" National Statistical Office 2012

34 Tyroler HA., "Coronary heart disease epidemiology in the 21st century" 22 : 7-13, 2000

35 Singleton VL, "Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents" 16 : 144-158, 1965

36 Uto-Kondo H, "Coffee consumption enhances high-density lipoprotein-mediated cholesterol efflux in macrophages" 106 : 779-787, 2010

37 Kim BK, "Cholesterol-lowering effect of mushroom powder in hyperlipidemic rats" 30 : 510-515, 2001

38 Ngamukote S, "Cholesterol-lowering activity of the major polyphenols in grape seed" 17 : 5054-5061, 2011

39 Miettinen TA., "Cholesterol absorption inhibition : A strategy for cholesterol-lowering therapy" 55 : 710-716, 2001

40 Seeram NP, "Blackberry, black raspberry, blueberry, cranberry, red raspberry and strawberry extracts inhibit growth and stimulated apoptosis of human cancer cells in vitro" 54 : 9329-9339, 2006

41 Wang SY, "Antioxidant activity in fruits and leaves of blackberry, raspberry, and strawberry varies with cultivar and developmental stage" 48 : 140-146, 2000

42 Aiello RJ, "ABCA1-deficient mice : Insights into the role of monocyte lipid efflux in HDL formation and inflammation" 23 : 972-980, 2003

43 Steinberg D., "A docking receptor for HDL cholesterol esters" 271 : 460-461, 1996