Determination of Aflatoxins Using High-Performance Liquid Chromatography with Optimized Fluorescence Detection

국문 초록 (Abstract)

HPLC에 의한 주요 aflatoxins(afatoxin B₁, B₂, G₁ 및 G₂)의 동시 분석에서 postcolumn 유도체화법을 시도하였다. Electrochemical cell(Kobra-cell)을 사용한 postcolumn 유도체화법은 기존의 precolumn 유도체화법보다 분석시간을 단축하였으며(약 1/2 단축), 더 안전하고, 향상된 분석능을 보였다. Aflatoxin B₁과 G₁의 경우 10~100 ppb에서, 그리고 B₂와 G₂의 경우 3~30 ppb에서 직선성을 나타내었다. Aflatoxin B₁과 G₁은 각각 88.9% 및 100.5%로 양호환 회수육을 보였다. Aflatoxin B₂와 G₂의 경우 분리도는 우수하였으나 회수율에 있어서 변이가 크게 나타났다.

다국어 초록 (Multilingual Abstract)

A postcolumn derivatization method was tried for the simultaneous determination of four major aflatoxins (B₁, B₂, G₁, and G₂) by high-performance liquid chromatography with fluorescence detection. As compared with a previous precolumn derivatization method, it was found that the postcolumn derivatization combined with an electrochemical cell (Kobra cell) was less time-consuming, safer, improved the sensitivity and selectivity, and provided good recoveries for aflatoxin B₁, (88.9% ) and G₁(100.5%). This method showed linearity from 10~100 ppb for aflatoxin B₁ and G₁, and from 3~30 ppb for aflatoxin B₂ and G₂. However, aflatoxin B₂ and G₂ were not detected satisfactorily although they showed good resolution.