국내산 봉독에 의한 BV-2 세포주에서 Nitric Oxide와 Tumor Necrocis Factor-α 생성에 미치는 항신경염증 작용에 관한 연구

다국어 초록 (Multilingual Abstract)

Inflammation in the brain has known to be associated with the development of a various neurologiacal diseases. The hallmark of neuro-inflammation is the activation of microglia, brain macrophage. Proinflammatory compounds including nitric oxide (NO) and tumor necrosis factor-α (TNF-α) are the main cause of neuro-degenerative disease such as Alzheimer's disease. We showed that honeybee venom (KBV) produced and purified in Korea regulated lipopolysaccharides (LPS) induced nitric oxide and TNF-α in the murine microglia, BV-2 cell line. The production of proinflammatory cytokines, NO and TNF-α were examined by LPS in BV-2 cell. BV-2 cells activated with LPS were treated with various doses of KBV. Supernatants were analyzed for the production of NO and TNF-α using Griess reagent and enzyme-linked immunosorbent assay (ELISA), respectively. KBV up to 100 ng/㎖ still required to inhibit NO and TNF-α induced by LPS. These results suggest that KBV has an anti-inflammatory effect by inhibiting NO and TNF-α expression. KBV could be applied useful for inhibiting the production of NO production in neurodegenerative diseases. In addition, the pharmacological aspects of the individual components of KBV are recommended for future trials