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다국어 초록 (Multilingual Abstract)

Background: Even though the modulatory effects of Magnisum (Mg) and its alloys on bone-healing cells have been widely investigated during the last two decades, relatively limited attention has been paid on their inflammationmodulatory properties. Understanding the activation process of macrophages in response to the dynamic degradation process of Mg as well as the relationship between macrophage phenotypes and their osteogenic potential is critical for the design and development of advanced Mg-based or Mg-incorporated biomaterials. Methods: In this work, a Ti-0.625 Mg (wt.%) alloy fabricated by mechanical alloying (MA) and subsequent spark plasma sintering (SPS) was employed as a material model to explore the inflammatory response and osteogenic performance in vitro and in vivo by taking pure Ti as the control. The data analysis was performed following Student’s t-test. Results: The results revealed that the macrophages grown on the Ti-0.625 Mg alloy underwent sequential activation of M1 and M2 phenotypes during a culture period of 5 days. The initially increased environmental pH (~ 8.03) was responsible for the activation of M1 macrophages, while accumulated Mg2+ within cells contributed to the lateral M2 phenotype activation. Both M1 and M2 macrophages promoted osteoblast-like SaOS-2 cell maturation. In vivo experiment further showed the better anti-inflammatory response, regenerative potentiality and thinner fibrous tissue layer for the Ti-0.625 Mg alloy than pure Ti. Conclusion: The results highlighted the roles of Mg degradation in the Ti-0.625 Mg alloy on the sequential activation of macrophage phenotypes and the importance of modulating M1-to-M2 transition in macrophage phenotypes for the design and development of inflammation-modulatory biomaterials.

참고문헌 (Reference)

1 Lv L, "Unveiling the mechanism of surface hydrophilicity-modulated macrophage polarization" 7 (7): 1800675-, 2018

2 Kawasaki T, "Toll-like receptor signaling pathways" 5 : 461-, 2014

3 McKiel LA, "The role of toll-like receptor signal-ing in the macrophage response to implanted materials" 1-14, 2019

4 Chen Z, "The effect of osteoim-munomodulation on the osteogenic effects of cobalt incorporated β-tricalcium phosphate" 61 : 126-138, 2015

5 Huang Q, "The design, development, and in vivo performance of intestinal anastomosis ring fabricated by magnesium zinc strontium alloy" 106 : 110158-, 2020

6 Huang Q, "The cu-containing TiO2 coatings with modulatory effects on macrophage polarization and bactericidal capacity prepared by micro-arc oxidation on titanium sub-strates" 170 : 242-250, 2018

7 Jin L, "The bioeffects of degradable products derived from a biodegradable Mg-based alloy in macrophages via heterophagy" 106 : 428-438, 2020

8 Anderson JM, "The Immune Response to Implanted Materi-als and Devices" Springer 15-36, 2017

9 Wang YN, "Texture analysis in hexagonal materials" 81 (81): 11-26, 2003

10 Qiao W, "TRPM7 kinase-mediated immunomodulation in macrophage plays a central role in magnesium ion-induced bone regeneration" 12 (12): 2885-, 2021