Antioxidant activity of melanin related products obtained from enzymatic oxidation of catechin was studied from the catechin-polyphenol oxidase reaction at pH 6.5 and 25℃ at various time intervals. All catechin-enzyme reaction products (CERPs) were ...
Antioxidant activity of melanin related products obtained from enzymatic oxidation of catechin was studied from the catechin-polyphenol oxidase reaction at pH 6.5 and 25℃ at various time intervals. All catechin-enzyme reaction products (CERPs) were brown in varied intensities with increased absorption at 210-220, 380-390nm, and 420-430 nm. CERPs obtained at the early stage of the reaction showed a higher antioxidant activity than those from the later stage. Antioxidant activity of CERPs may be explained by their abilities of hydrogen atom donation, free radical scavenging, and lipoxygenase inhibition. However, the exact mechanism of the antioxidant activity of CERPs cannot be explained until their chemical structures are elucidated.