Hedera helix의 組織培養에 關한 硏究 : Ⅰ. Callus 形成 및 器官分化에 미치는 生長調節物質의 影響 Ⅰ. Effects of Growth Regulators on the Callus Formation and Organogenesis = Studies on the Tissue Culture of Hedera helix

국문 초록 (Abstract)

Hedera helix의 葉柄과 Leaf disc를 切取하여 生長調節物質을 달리한 Blayde's 基本培地에 培養하였던바, 그 結果를 要約하면 다음과 같다.
1. NAA 2㎎/ℓ+Kinetin 2㎎/ℓ에서 Callus의 形成이 가장 旺盛하였다.
2. 誘起된 Callus로부터 不定根의 發生은 NAA 5㎎/ℓ單獨處理에서 가장 효과가 있었다.
3. 不定根의 發生樣相은 Leaf disc에서는 多束狀으로 葉柄에서는 산발적으로 나타났다.
4. 2,4-D가 組合된 培地에서 Callus의 褐變現象이 나타났으며, 2,4-D의 濃度가 높을수록 현저했다.

다국어 초록 (Multilingual Abstract)

The leaf petioles and discs of Hedera helix were cultured on the Blayde's basic medium supplemented with various combinations of growth regulators.
The results obtained are summarized as follows :
1. The calluses were induced vigorously from the Blayde's medium supplemented with 2㎎/1 NAA and 2㎎/1 kinetin.
2. The best growth of adventitous roots appeared on the calluses that had formed on the media cantaining only 5㎎/1 NAA.
3. Multiple roots were formed on the calluses of leaf disc, whereas single roots on the those of leaf petiole.
4. Brownish calluses were observed on the media with 2, 4-D.