복분자의 항산화 및 광독성 억제활성에 미치는 영향 = The Effects of Anti-Oxidant and Phototoxic Inhibitory Activity of Rubus coreanus Fruits

다국어 초록 (Multilingual Abstract)

Objectives : Oxidation by active oxygen in the body, phototoxicity and photoallergic interest has grown, antioxidant and phototoxicity inhibiting substances for research progress. The purpose of this study was to examine the effects on antioxidant and phototoxicity of Rubus coreanus Fruits. Meterial and Methods : Hs68 cell lines using the DPPH radical scavenging activity, cytotoxic, phototoxic inhibitory activity and apoptosis were measured. Results : 1. In MTT assay, the concentrations of Rubus coreanus Fruits that were used on the test had no cytotoxicity. 2. In DPPH radical scavenging activity, the concentration of $50{\mu}g/ml$, $100{\mu}g/ml$ anti-oxidant effect of Rubus coreanus Fruits was statistically significant increased than control group in dose-dependantly. 3. In phototoxic inhibitory activity, Rubus coreanus Fruits dose-dependently increased the cell viability of Hs68 cell lines. 4. The concentration of $50{\mu}g/ml$ Rubus coreanus Fruits inhibited the enrichment of nucleus in the Hs68 cell stimulated with UVB. Conclusions : These results indicate that Rubus coreanus Fruits has anti-oxidant effects and Phototoxic Inhibitory Activity. If further study is performed, the use of Rubus coreanus Fruits will be valuable and beneficial in the therapy of skin aging and damage.

참고문헌 (Reference)

1 전영선, "콜라겐 섭취가 안면피부 주름에 미치는 영향" 주식회사 한국피부임상과학연구소 7 (7): 79-94, 2009

2 윤유식, "연근별 황기의 항산화 및 항당뇨 활성 평가 고찰" 한국생약학회 40 (40): 1-5, 2009

3 김설미, "안면 피부관리와 항산화 비타민 섭취의 피부건강 상태 변화" 주식회사 한국피부임상과학연구소 7 (7): 111-125, 2009

4 신현재, "복분자 유용 성분의 연구과 그 응용" 한국생물공학회 21 (21): 405-409, 2006

5 유진균, "목단피 물 추출물의 항산화 및 Tyrosinase 억제효과" 한국식품영양과학회 38 (38): 292-296, 2009

6 임현정, "丹梔逍遙散이 자외선을 조사한 피부진피세포의 활성 및 유전자발현에 미치는 영향" 대한한방부인과학회 24 (24): 13-32, 2011

7 Lee KH, "extract promotes osteoblast differentiation and inhibits bone-resorbing mediators in MC3T3-E1 cells" 34 (34): 643-654, 2006

8 Ritte L, "UV-light-induced signal cascades and skin aging" 1 (1): 705-720, 2002

9 Laure R, "UV-light-induced signal cascades and skin aging" 1 : 705-720, 2002

10 B Halliwell, "The deoxyribose method : A simple"test-tube"assay for determination of rate constants for reactions of hydroxyl radicals" 165 : 215-219, 1987

11 N Ramarathnam, "The contribution of plant food antioxidants to human health" 6 : 75-82, 1995

12 Nam JH, "The anti-gastropathic and anti-rheumatic effect of niga-ichigoside F1 and 23-hydroxytormentic acid isolated from the unripe fruits of Rubus coreanus in a rat model" 29 (29): 967-970, 2006

13 Lee YA, "Tannins from Rubus coreanus" 26 (26): 27-30, 1995

14 Latt SA, "Spectral studies on 33258Hoechst and related bisbenzimidazole dyes useful for fluorescent detection of deoxyribonucleic acid synthesis" 24 (24): 24-33, 1976

15 Debacq-Chainiaux F, "Repeated exposure of human skin fibroblasts to UVB at subcyto-toxic level triggers premature sensecence through the TGF-betal signaling pathway" 118 (118): 743-758, 2005

16 Latt SA, "Recent developments in the detection of deoxyribonucleic acid synthesis by 33258 Hoechst fluorescence" 23 (23): 493-505, 1975

17 Mosmann T, "Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays" 65 (65): 55-63, 1983

18 Cimino F, "Protective effects of a red orange extract on UVB-induced damage in human keratinocytes" 30 (30): 129-138, 2007

19 Lee MW, "Phenolic compounds from the leaves of Rubus coreanus" 39 : 200-204, 1995

20 Uitto J, "Molecular mechanism of cutaneous aging" 21 : 614-622, 1989

21 Park JH, "Induction of heme oxygenase-1 mediates the antiinflammatory effects of the ethanol extract of Rubus coreanus in murine macrophages" 351 (351): 146-152, 2006

22 Chen VL, "Immunochemistry of elastotic material in sun-damaged skin" 87 (87): 334-337, 1986

23 "Hoechst and related bisbenzimidazole dyes useful for fluorescent detection of deoxyribonucleic acid synthesis" 24 (24): 24-33,

24 The National College of Oriental Medicine Herbology Classroom, "Herbology" Youngrimsa 694-695, 2006

25 이지해, "Hairless mice에서의 대두 홍삼 혼합 분말(APB-03)의 경구 반복 투여 시 피부 주름 생성 예방 효과" 한국식품과학회 37 (37): 989-996, 2005

26 Mantena SK, "Grape seed proanthocyanidins inhibit UV-radiationinduced oxidative stress and activation of MAPK and NF-kappaB signaling in human epidermal keratinocytes" 40 (40): 1603-1614, 2006

27 Moon JH, "Functional Components and Physiological Activity of Tea" 1 : 177-193, 1995

28 Darr D, "Free radicals in cutaneous biology" 102 (102): 671-675, 1994

29 Kim MS, "Flavonoids from the leaves of Rubus coreanus" 41 : 1-6, 1997

30 Moysan A, "Effects of ultraviolet A and antioxidant defense in cultured fibroblasts and Keratinocytes" 11 : 192-197, 1996

31 Youn JI, "Effects of Ultraviolet Radiation on the Skin" 20 (20): 181-186, 1995

32 Stempel E, "Drug-induced photosensitivity" 13 (13): 200-204, 1973

33 Wiseman H, "Dietary influences on membrane function : importance in protection against oxidative damage and disease" 7 (7): 2-15, 1996

34 Schwartz E, "Collagen alteration in chronically sun damaged human skin. Photochem" 58 (58): 841-844, 1993

35 Lee YS, "Clinical Dermatology" Ryo Moon Gak P. Co. 296-297, 1998

36 EN Frankel, "Antioxidants in lipid foods and their impact on food quality" 57 : 51-55, 1996

37 Shindo Y, "Antioxidant defense mechanisms in murine epidermis and dermis and their responses to ultraviolet light" 100 (100): 260-265, 1993

38 Wang SY, "Antioxidant activity in fruits and leaves of blackberry, raspberry, and strawberry varies with cultivar and developmental stage" 48 (48): 140-146, 2000

39 Wada L, "Antioxidant activity and phenolic content of Oregon caneberries" 50 : 3495-3500, 2002

40 Choi J, "Antinociceptive and antiinflammatory effects of Niga-ichigoside F1and 23-hydroxytormentic acid obtained from Rubus coreanus" 26 (26): 1436-1441, 2003

41 Shin TY, "Action of Rubus coreanus extract on systemic and local anaphylaxis" 16 (16): 508-513, 2002

42 Lim HW, "Abnormal responses to ultraviolet radiation : photosensitivity induced by exogenous agents. In: Fitzpatrick's Dermatology in General Medicine" McGraw-Hill 1-828, 2008

43 Ferguson J, "A study of benoxaprofen-induced photosensitivity" 107 (107): 429-442, 1982

44 Yaar M, "A cellular and molecular mechanism cutaneous aging" 16 (16): 915-922, 1990