반응표면분석법을 이용한 댕댕이 기능성성분의 마이크로웨이브추출조건 최적화 = Optimization of microwave-assisted extraction process for blue honeysuckle (Lonicera coerulea L.) using response surface methodology

다국어 초록 (Multilingual Abstract)

Functional compounds including flavonoids, anthocyanins, polyphneols and antioxidants were extracted from blue honeysuckle (Lonicera caerulea L.) using highly efficient microwave-assisted extraction. And extraction process was modeled and optimized according to response surface methodology (RSM). The independent variables ($X_n$) were ethanol concentration ($X_1$: 0, 25, 50, 75, 100%), irradiation time ($X_2$: 1, 3, 5, 7, 9 min), and microwave power ($X_3$: 60, 120, 180, 240, 300 W). Dependent variables ($Y_n$) were total flavonoid contents ($Y_1$), total anthocyanin contents ($Y_2$), total polyphenol contents ($Y_3$) and antioxidant activity ($Y_4$). Four-dimensional response surface plots were generated based on the fitted second-order polynomial models to get optimal conditions. Estimated optimal conditions for 4 responses were ethanol concentration of 54-72%, irradiation time of 7.1-7.6 min, and microwave power of 243-251 W. Ridge analysis predicted the maximal responses of total flavonoid content, total anthocyanin content, total polyphenol content and antioxidant activity were 38.00 mg RE/g, 6.80 mg CGE/g, 14.90 mg GAE/g, 89.10%, respectively. Verification experiment was carried out at predicted optimal conditions and experimental values for total flavonoid content, total anthocyanin content, total polyphenol content and antioxidant activity were 38.10 mg RE/g, 6.72 mg CGE/g, 14.91 mg GAE/g and 89.13%, respectively. No significant difference was observed between predicted and experimental values, indicating good fitness of fitted model and successful application of RSM.

참고문헌 (Reference)

1 안봉전, "한국산 감잎로부터 Polyphenol계 생리활성물질 분리" 한국응용생명화학회 45 (45): 212-217, 2002

2 이은진, "포도씨 카테킨류의 마이크로웨이브 추출특성" 한국식품과학회 40 (40): 510-515, 2008

3 이은진, "포도씨 추출물의 총 카테킨 함량과 전자공여능에 대한 마이크로웨이브 추출조건 최적화" 한국식품저장유통학회 15 (15): 840-846, 2008

4 강병태, "자두와인 내 폴리페놀 화합물의 항산화 및 항바이러스 활성" 한국식품저장유통학회 15 (15): 891-896, 2008

5 김현구, "반응표면분석을 이용한 양파의 추출효율 및 기능성에 대한 모니터링" 한국식품영양과학회 36 (36): 105-110, 2007

6 홍주헌, "반응표면분석에 의한 Acai(Euterpe oleracea Mart.) 기능성분의 추출조건 최적화" 동아시아식생활학회 21 (21): 713-722, 2011

7 김대익, "반응표면분석법을 이용한 야관문(Lespedeza cuneata) 기능성분의 에탄올 추출조건 최적화" 한국식품조리과학회 28 (28): 275-283, 2012

8 권중호, "마이크로파 추출공정에 의한 쥐깨풀 유용성분의 추출조건 최적화" 한국식품저장유통학회 12 (12): 617-623, 2005

9 이혜진, "마이크로웨이브 추출조건에 따른 참나무 겨우살이 추출물의 생리활성" 한국식품저장유통학회 18 (18): 72-78, 2011

10 주은영, "마이크로웨이브 추출조건에 따른 백지 추출물의 폴리페놀 함량과 항산화 작용" 한국식품저장유통학회 15 (15): 133-138, 2008

11 최근호, "급성 알코올 중독에서 헛개나무 추출물을 포함한 식품 조성물의 보호 효과" 한국식품영양과학회 40 (40): 1107-1112, 2011

12 이동성, "가열 추출법과 마이크로웨이브 추출법을 이용한 황금 물 추출물의뇌세포 보호 및 항산화 효과 비교 연구" 한국생약학회 45 (45): 55-61, 2014

13 Truong VD, "Pressurized liquid extraction and quantification of anthocyanins in purple-fleshed sweet potato genotypes" 26 : 96-103, 2012

14 Hui Teng, "Optimization of Microwave-assisted Extraction of Active Components from Chinese Quince using Response Surface Methodology" 한국응용생명화학회 52 (52): 694-701, 2009

15 Pare JRJ, "Microwave- assisted natural products extraction"

16 Ahmad N, "Green tea polyphenol epigallocatechin-3-gallate differentially modulates nuclear factor κB in cancer cells versus normal cells" 376 : 338-346, 2000

17 Eum JH, "Food composition for improving liver functioncomprising a Lonicera caerulea L. var. edulis extract"

18 Svarcova I, "Berry fruits as a source of biologically active compounds: the case of Lonicera caerulea" 151 : 163-174, 2007

19 Blois MS, "Antioxidant determinations by the use of a stable free radical" 181 : 1199-1200, 1958

20 Schauss AG, "Antioxidant capacity and other bioactivities of the freeze-dried Amazonian palm berry, Euterpe oleraceae Mart. (Acai)" 54 : 8604-8610, 2006

21 Singleton VL, "Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent" 299 : 152-178, 1999

22 Cho SH, "A pharmaceutical health food for preventing or improving the brain ischemia disease containing extract of Lonicera caerulea L. var. edulis fruits"