닭의 간과 적혈구의 핵 단백질의 비교연구

국문 초록 (Abstract)

닭 간 HMG단백질을 산추출 CM Sephadex C25, Bio Rex 70을 이용하여 분리 정제하였으며 분자량은 HMG1단백질이 29,000 HMG2단백질이 27,000이었다. 이것은 적혈구 HMG단백질의 분자량과 동일하였으며 전기영동에서도 동일한 이동도로 나타났고 면연학적 검토에서도 동일한 항원성을 가지는 단백질로 판명되었다. 그러나 아미노산 분석과 peptide map의 결과 간HMG단백질과 적혈구HMG단백질은 아미노산 조성면에서 약간의 차이를 보였다. 이 결과 간HMG단백질과 적혈구HMG단백질은 성질이 유사하면서도 각각 다른 단백질로 판명되었다.

다국어 초록 (Multilingual Abstract)

Nuclei proteins were purified from chick liver to homogeneity by means of acid extraction, CM Sephadex C 25 column chromatography and Bio Rex 70 column chromatography. The molecular weights of liver Nuclei proteins 1 and 2, as estimated by electrophoresis on SDS-polyacrylamide gel, are 29,000 and 27,000, respectively. These molecular weights are identical with those of Nuclei Proteins 1 and 2 isolated from chick erythrocyte. The liver and erythrocyte Nuclei Proteins also co-migrated in acetic acid-urea gel electrophoresis. Furthermore, the antisera, raised against liver Nuclei Proteins 1 and 2, cross-reacted with erythrocyte Nuclei Proteins 1 and 2, respectively. However, the amino acid compositions of liver Nuclei Proteins 1 and 2 were found to be different from those of corresponding erythrocyte Nuclei proteins ; the contents of serine and proline in liver Nuclei proteins were higher than those in erythrocyte Nuclei proteins while the content of lysine in liver Nuclei proteins was lower than the erythrocyte Nuclei proteins. These results suggest that, in spite of similarities in many respects, the liver and erythrocyte Nuclei proteins in chicks are different proteins.

목차 (Table of Contents)

• Abstract
  
• 서론
  
• 재료 및 방법
  
• 결과 및 고찰
  
• 요약
  
• 문헌