가감대황목단피탕(加減大黃牧丹皮湯)의 급성 췌장염 억제 효과 = Inhibitory effect of acute pancreatitis by Gagam-daewhangmokdanpi-tang

다국어 초록 (Multilingual Abstract)

Objectives : In this study, we investigated the effects of Daehwangmokdanpitang-gagambang(DMG) on treatment for cholecystokinin-octapeptide(CCK)-induced acute pancreatitis(AP) in rats. Methods : Male Wistar rats weighing 200 to 250 g were divided into three groups. The first was normal untreated group, the second was in treatment with DMG group; DMG was administered orally, followed by 75 ${\mu}g/kg$ CCK subcutaneously three times, after 1, 3 and 5 h. This whole procedure was repeated for 5 days. The third was in treatment with saline group, the protocol was the same as in treatment group with DMG. We determined the pancreatic weight/body weight ratio, the levels of pancreatic HSP60, HSP72, ERK, p38 MAPK and the secretion of pro-inflammatory cytokines. Repeated CCK treatment resulted in the typical laboratory and morphological changes of experimentally induced pancreatitis. Results : DMG was significantly decreased the pancreatic weight/body weight ratio in CCKinduced AP and DMG increased HSP60 and HSP72 compared with CCK-induced AP. DMG suppressed ERK and p38 MAPK activation. Additionally, the secretion of $IL-1{\beta}$ and $TNF-{\alpha}$ and the levels of amylase and lipase were lower than that saline. Conclusions : DMG has an effect to treatment for CCK-induced AP.

참고문헌 (Reference)

1 심찬섭, "복부초음파진단학" 서울: 여문각 277-, 2000

2 이장훈, "동의임상내과학" 서울: 법인문화사 426-438, 1999

3 Lee H, "Water immersion stress induces heat shock protein 60 expression and protects against pancreatitis in rats" 119 : 220-229, 2000

4 Rakonczay Z. Jr,, "Water immersion pretreatment decreases pro-inflammatory cytokine production in cholecystokinin-octapeptide-induced acute pancreatitis in rats possible role of HSP72" 17 : 520-535, 2001

5 Norman J, "The role of cytokines in the pathogenesis of acute pancreatitis" 175 : 76-83, 1998

6 Lane JS.,, "Platelet activating factor antagonism reduces the systemic inflammatory response in a murine model of acute pancreatitis" 99 : 365-370, 2001

7 Dabrowski A, "Jun kinases are rapidly activated by cholecystokinin in rat pancreas both in vitro and in vivo" 271 : 5686-5690, 1996

8 Exley AR, "Endotoxaemia and serum tumour necrosis factor as prognostic markers in severe acute pancreatitis" 1126-8, 1992;33

9 Okuyama A, "Differential induction of HSP60 and HSP72 by different stress situations in rats" 42 : 1473-1479, 1997

10 Panozzo MP, "Diagnostic utility of a new monoclonal antibody pancreatic isoamylase assay in chronic pancreatic diseases" 485-8, 1990;28

11 Panozzo MP, "Diagnostic utility of a new monoclonal antibody pancreatic isoamylase assay in chronic pancreatic diseases" 485-8, 1990;28

12 Pagani F, "Clinical and analytical evaluation of a continuous enzymatic method for measuring pancreatic lipase activity" bo (bo): 304-8, 1993;39

13 Pagani F, "Clinical and analytical evaluation of a continuous enzymatic method for measuring pancreatic lipase activity" bo (bo): 304-8, 1993;39

14 Fink-Bennet, "Cholecystokinin cholescintigraphy:Detection of abnormal gallbladder motor fuction in patients with chronic acalculous gallbladder diseaes" Cholecystokinin cholescintigraphy 1695-9, 1991;32

15 Dabrowski A, "Cholecystokinin and EGF activate a MAPK cascade by different mechanisms in rat pancreatic acinar cells" 273 : 1472-1479, 1997

16 Strowski MZ, "Caerulein pancreatitis increases mRNA but reduces protein levels of rat pancreatic heat shock proteins" 273 : 937-945, 1997

17 Lampel M, "Acute interstitial pancreatitis in the rat induced by excessive doses of a pancreatic secretagogue" 97-117, 1977;373

18 Krishnamurthy GT, "A tecknique for quantitative analysis of motor function of the human gallbladder" 482-90, 1981;80