Identification of the unfolded protein response (UPR)-related genes from Bombyx mori cell lines by a subtractive hybridization approach

다국어 초록 (Multilingual Abstract)

The baculovirus expression vector system (BEVS) is one of the powerful insect cell systems for heterologous protein expression. However, over-expression of heterologous proteins in this system sometimes results in protein misfolding and aggregation because of insufficient levels of folding catalysts.
In previous study using the differential screening (DS) method,we isolated only 40 differentially expressed genes after treatment with tunicamycin, an unfolded protein response (UPR)inducer. To isolate more protein folding catalysts from insect,we performed suppressive subtractive hybridization (SSH)with untreated and tunicamycin-treated Bm5 cell lines in this study. We could isolate 366 differentially expressed clones by SSH method and produced expressed sequence tags (ESTs).
ESTs included the UPR pathway-related genes involved in protein folding, including heat shock proteins, molecular chaperones,foldases, as well as glycosylation and secretory pathway related genes. Identification of the tunicamycin responsive genes using SSH provides more information about the UPR-related genes in insect cells, and will facilitate modifications of the protein folding pathway in the ER to improve heterologous protein expression.

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