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      KCI등재 SCOPUS SCIE

      Identification of the unfolded protein response (UPR)-related genes from Bombyx mori cell lines by a subtractive hybridization approach

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      https://www.riss.kr/link?id=A103732234

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      다국어 초록 (Multilingual Abstract)

      The baculovirus expression vector system (BEVS) is one of the powerful insect cell systems for heterologous protein expression. However, over-expression of heterologous proteins in this system sometimes results in protein misfolding and aggregation because of insufficient levels of folding catalysts.
      In previous study using the differential screening (DS) method,we isolated only 40 differentially expressed genes after treatment with tunicamycin, an unfolded protein response (UPR)inducer. To isolate more protein folding catalysts from insect,we performed suppressive subtractive hybridization (SSH)with untreated and tunicamycin-treated Bm5 cell lines in this study. We could isolate 366 differentially expressed clones by SSH method and produced expressed sequence tags (ESTs).
      ESTs included the UPR pathway-related genes involved in protein folding, including heat shock proteins, molecular chaperones,foldases, as well as glycosylation and secretory pathway related genes. Identification of the tunicamycin responsive genes using SSH provides more information about the UPR-related genes in insect cells, and will facilitate modifications of the protein folding pathway in the ER to improve heterologous protein expression.
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      The baculovirus expression vector system (BEVS) is one of the powerful insect cell systems for heterologous protein expression. However, over-expression of heterologous proteins in this system sometimes results in protein misfolding and aggregation be...

      The baculovirus expression vector system (BEVS) is one of the powerful insect cell systems for heterologous protein expression. However, over-expression of heterologous proteins in this system sometimes results in protein misfolding and aggregation because of insufficient levels of folding catalysts.
      In previous study using the differential screening (DS) method,we isolated only 40 differentially expressed genes after treatment with tunicamycin, an unfolded protein response (UPR)inducer. To isolate more protein folding catalysts from insect,we performed suppressive subtractive hybridization (SSH)with untreated and tunicamycin-treated Bm5 cell lines in this study. We could isolate 366 differentially expressed clones by SSH method and produced expressed sequence tags (ESTs).
      ESTs included the UPR pathway-related genes involved in protein folding, including heat shock proteins, molecular chaperones,foldases, as well as glycosylation and secretory pathway related genes. Identification of the tunicamycin responsive genes using SSH provides more information about the UPR-related genes in insect cells, and will facilitate modifications of the protein folding pathway in the ER to improve heterologous protein expression.

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      참고문헌 (Reference)

      1 Liu CY, "The unfolded protein response" 116 : 1861-1862, 2003

      2 Schroder M, "The mammalian unfolded protein response" 74 : 739-789, 2007

      3 Diatchenko L, "Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries" 93 : 6025-6030, 1996

      4 Ron D, "Signal integration in the endoplasmic reticulum unfolded protein response" 8 : 519-529, 2007

      5 Sitia R, "Quality control in the endoplasmic reticulum protein factory" 426 : 891-894, 2003

      6 Leal WS, "Olfactory proteins mediating chemical communication in the navel orangeworm moth, Amyelois transitella" 4 : 7235-, 2009

      7 Whittaker RH, "New concepts of kingdoms of organisms" 163 : 150-160, 1969

      8 Ailor EM, "Modifying secretion and posttranslational processing in insect cells" 10 : 142-145, 1999

      9 Trask DK, "Keratins as markers that distinguish normal and tumor-derived mammary epithelial cells" 87 : 2319-2323, 1990

      10 Hoog C, "Isolation of a large number of novel mammalian genes by a differential cDNA library screening strategy" 19 : 6123-6127, 1991

      1 Liu CY, "The unfolded protein response" 116 : 1861-1862, 2003

      2 Schroder M, "The mammalian unfolded protein response" 74 : 739-789, 2007

      3 Diatchenko L, "Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries" 93 : 6025-6030, 1996

      4 Ron D, "Signal integration in the endoplasmic reticulum unfolded protein response" 8 : 519-529, 2007

      5 Sitia R, "Quality control in the endoplasmic reticulum protein factory" 426 : 891-894, 2003

      6 Leal WS, "Olfactory proteins mediating chemical communication in the navel orangeworm moth, Amyelois transitella" 4 : 7235-, 2009

      7 Whittaker RH, "New concepts of kingdoms of organisms" 163 : 150-160, 1969

      8 Ailor EM, "Modifying secretion and posttranslational processing in insect cells" 10 : 142-145, 1999

      9 Trask DK, "Keratins as markers that distinguish normal and tumor-derived mammary epithelial cells" 87 : 2319-2323, 1990

      10 Hoog C, "Isolation of a large number of novel mammalian genes by a differential cDNA library screening strategy" 19 : 6123-6127, 1991

      11 Zhang K, "Identification and characterization of endoplasmic reticulum stress-induced apoptosis in vivo" 442 : 395-419, 2008

      12 Ashburner M, "Gene ontology: tool for the unification of biology. The Gene Ontology Consortium" 25 : 25-29, 2000

      13 Yun EY, "Expression of antibacterial protein, nuecin, using baculovirus expression vector system in Bm5 insect cell and Bombyx mori" 44 : 69-73, 2002

      14 Kuang WW, "Differential screening and suppression subtractive hybridization identified genes differentially expressed in an estrogen receptor-positive breast carcinoma cell line" 26 : 1116-1123, 1998

      15 Watson JB, "Differential cDNA screening strategies to identify novel stage-specific proteins in the developing mammalian brain" 15 : 77-86, 1993

      16 Goo TW, "Construction of the cDNA library and selection of differentially expressed clones from Bombyx mori Bm5 cell line treated with tunicamycin" 23 : 183-190, 2001

      17 Lee SH, "Comparative analysis of sequences expressed during the liquid-cultured mycelia and fruit body stages of Pleurotus ostreatus" 35 : 1115-1135, 2002

      18 Yokoyana N, "Co-expression of human chaperone Hsp70 and Hsdj or Hsp40 co-factor increases solubility of overexpressed target proteins in insect cells" 1493 : 119-124, 2000

      19 Yun EY, "Changes in cellular secretory processing during baculovirus infection" SPRINGER 27 : 1041-1045, 2005

      20 Jarvis DJ, "Biosynthesis and processing of the Autographa californica nuclear polyhedrosis virus gp64 protein" 205 : 300-313, 1994

      21 Ewing B, "Base-calling of automated sequencer traces using phred. I. Accuracy assessment" 8 : 175-185, 1998

      22 Possee RD, "Baculoviruses as expression vectors" 8 : 569-572, 1997

      23 Summers MD, "A manual of methods for baculovirus vectors and insect cell culture procedures" 1555 : 1987

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      학술지 이력

      학술지 이력
      연월일 이력구분 이력상세 등재구분
      2023 평가예정 해외DB학술지평가 신청대상 (해외등재 학술지 평가)
      2020-01-01 평가 등재학술지 유지 (해외등재 학술지 평가) KCI등재
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      2004-01-01 평가 등재학술지 선정 (등재후보2차) KCI등재
      2003-01-01 평가 등재후보 1차 PASS (등재후보1차) KCI등재후보
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      학술지 인용정보

      학술지 인용정보
      기준연도 WOS-KCI 통합IF(2년) KCIF(2년) KCIF(3년)
      2016 0.51 0.12 0.38
      KCIF(4년) KCIF(5년) 중심성지수(3년) 즉시성지수
      0.32 0.27 0.258 0.02
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