대동맥편 및 폐동맥편 내피세포의 생육성판정에 관한 연구 = Viability of Endothelial Cells using Flow Cytometry in Aortic and Pulmonary Arterial Graft in Pig

다국어 초록 (Multilingual Abstract)

Human allograft valves have been widely applied for the treatment of acquired and congenital cardiac diseases. One factor that some investigators consider to be important in determining allograft durability is the degree of cellular viability. Allografts comprise a heterogenous population of cells and determination of graft viability logically requires understanding viability of those specific populations.
This study was performed to quantify the viability of endothelial cells of allograft cardiac valves after 4℃ fresh preservation and -196℃ cryopreservation for 14 days using metabolic assay technique with ^(3)H-glycine and flow cytometric technique. To evaluate endothelial cell viability using Flow Cytometry, valved conduit were subjected to collagenase digestion. The resulting cell suspension was labeled with Griffonia simplicifolia agglutinin-fluorescein isothiocyanate (GSA-FITC), a marker for vascular endothelial cells. The cells were then incubated with propidium iodide (PI), which is excluded by viable cells. Flow cytometry evaluated endothelial cell viability by determination of percentage of GSA-FITC-positive cells that were negative for PI.
The metabolic assay shows that the viability of endithelial cells was 49% after cryopreservation and 17% after fresh preservation for 14 days.
The flow cytometric analysis shows that viability of endothelial cells was 39% after cryopreservation and 41% after fresh preservation for 14 days.
Flow cytometry is fast and precise quantitative method for evaluating the viability of endothelial cells.