油菜의 分離한 小胞子로부터 半數體 胚生産 = In vitro embryogenesis of haploids from isolated microspores of oil seed rape(Brassica napus L. ssp. oleifera)

다국어 초록 (Multilingual Abstract)

Microspore culture was carried out in order to compare the difference of embryo yield between spring type and winter type of Brassica napus L. and isolated microspores from three genotypes of winter type and three genotypes of spring type were cultured in modified Nitsch and Nitsch (NN) medium supplemented with 13% (w/v) sucrose, 0.05mg/ℓ BA and 1.0mg/ ℓ NAA. Temperature was 32.5℃ for 3 days of the initial stage in the dark and maintained at 25℃ in the dark. Embryo yields and frequency of normal embryo production showed remarkable contrast according to genotypes and growth habit. The highest frequency of embryogenesis was obtained from buds of 2.8~3.2mm in size approximately one week after first flowering and the buds of the sizes contained mainly microspores of late uninucleate stage. Embryos were induced from microspores isolated from the buds of 2.3~2.7mm, 2.8~3.2mm, 3.3~3.7mm and 3.8~4.2mm in the spring type. But only the buds of 2.3~2.7mm and 2.8~3.2mm were able to from embryos in the winter type.

목차 (Table of Contents)

• ABSTRACT
• 緖言
• 材料 및 方法
• 結果 및 考察
• 摘要
• 引用文獻