Elicited cells with methyl jasmonate continued to produce benzophenanthridine alkaloids throughout medium changes in suspension cultures of Eschscholtzia californica. Large increases in alkaloid production were observed by re-elicitations with medium ...
Elicited cells with methyl jasmonate continued to produce benzophenanthridine alkaloids throughout medium changes in suspension cultures of Eschscholtzia californica. Large increases in alkaloid production were observed by re-elicitations with medium changes. The total alkaloid production increased during the successive elicitation steps reaching a maximum level on the 4th elicitation. The highest total alkaloid produced was 250㎎/l, which was 2-fold higher than that of the single elicitation and 4-fold higher than that of the normal culture without elicitation. The large increases in alkaloid production in successive re-elicitations with medium changes are believed to be caused by the accumulation of the signal transduction compound, jasmonate.