<P><B>Purpose</B></P><P>In vaccine efficacy evaluation, visualization of pathogens in whole organism at each time point would be able to reduce the consuming animals and provide the <I>in vivo</I> information ...
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https://www.riss.kr/link?id=A107571180
2012
-
SCOPUS,KCI등재,ESCI
학술저널
83-87(5쪽)
0
상세조회0
다운로드다국어 초록 (Multilingual Abstract)
<P><B>Purpose</B></P><P>In vaccine efficacy evaluation, visualization of pathogens in whole organism at each time point would be able to reduce the consuming animals and provide the <I>in vivo</I> information ...
<P><B>Purpose</B></P><P>In vaccine efficacy evaluation, visualization of pathogens in whole organism at each time point would be able to reduce the consuming animals and provide the <I>in vivo</I> information within consistent background with identical organism.</P><P><B>Materials and Methods</B></P><P>Using IVIS spectrum whole live-animal imaging system, fluorescent intensity was optimized and visualized proportionately by concentrating <I>Escherichia coli</I> MC1061 strain which expresses GFP (<I>E. coli</I>-GFP) in BALB/C mice after injection.</P><P><B>Results</B></P><P>Local distribution of disseminated <I>E. coli</I>-GFP was traced in each organ by fluorescence. Detached organ showed more obvious fluorescent signal, and intestine showed strongest fluorescent signal.</P><P><B>Conclusion</B></P><P>This <I>in vivo</I> imaging method using GFP-tagged pathogen strain suggest quantified infected pathogens by fluorescence intensity in whole animals can provide the information about the localization and distribution after infection.</P>
Mucosal vaccine adjuvants update