Canine model of ischemic stroke with permanent middle cerebral artery occlusion: clinical features, magnetic resonance imaging, histopathology, and immunohistochemistry

다국어 초록 (Multilingual Abstract)

The purpose of this study was to identify time-related changes in clinical, MRI, histopathologic, and immunohistochemical findings associatedwith ischemic stroke in dogs. Additionally, the association of cerebrospinal fluid (CSF) and tissue levels of interleukin (IL)-6 with clinicalprognosis was assessed. Ischemic stroke was induced by permanent middle cerebral artery occlusion (MCAO) in nine healthy experimentaldogs. The dogs were divided into three groups according to survival time and duration of the experimental period: group A (survived only1 day), group B (1-week experimental period), and group C (2-week experimental period). Neurologic status was evaluated daily. Magneticresonance imaging (MRI) was performed according to a predetermined schedule. Concentration of IL-6 in CSF was measured serially afterischemic stroke. Postmortem examination was performed for all experimental dogs. During histopathological examination, variable degreesof cavitation and necrosis due to neuronal cytopathic effects, such as pyknotic nuclei and cytoplasmic shrinkage, were observed on the affectedside of the cerebral cortex in all dogs. Immunohistochemistry specific for IL-6 showed increased expression in the ischemic lesions. CSF IL-6concentrations and ischemic lesion volumes 1 day after ischemic stroke were significantly higher in group A compared to groups B and C.

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