Genus-Specific Polymerase Chain Reaction to Detect Mycoplasma Species

다국어 초록 (Multilingual Abstract)

Mycoplasmas are highly fastidious bacteria, difficult to culture and slow growing. Many species of mycoplasma are important pathogens causing respiratory infection in laboratory animals and known to affect influencing experimental results obtained with contaminated animals. Screening of Mycoplasma species is particularly desirable, because they are prevalent in commercial and research animal facilities. A consensus polymerase chain reaction (PCR) analysis was employed to detect Mycoplasma species and typing of the species was performed on the basis of sequence analysis of the PCR product. The target nucleic acid fragments were specifically amplified by consensus PCR with 16S ribosomal DNA primers. The sequencing analyses of amplified DNAs showed the effective differentiation of Mycoplasma species. Nasal swabs collected from rats were submitted to detect Mycoplasma species and three cases of Mycoplasma pulmonis were detected. In this study, the consensus PCR was able to detect successfully mycoplasma species. This consensus PCR was recommended for monitoring Mycoplasma species in laboratory animals. PCR and direct sequencing approach is effective for detecting and typing of Mycoplasma species.

목차 (Table of Contents)

• Materials and Methods
• Results
• Discussion
• Acknowledgments
• References