추백리가 감염된 닭의 간에서 발현이 증가하는 APOA1 단백질의 확인 = Identification of Upregulated APOA1 Protein of Chicken Liver in Pullorum Disease

다국어 초록 (Multilingual Abstract)

The aim of this study was to investigate differentially expressed proteins between normal chicken liver and chicken liver inffeted by Salmonella pullorum. 2-dimensional electrophoresis (2DE) and mass spectrometry (MS) were used to identify the proteins. More than 300 protein spots were detected on silver stained 2DE gels using pH 3$\~$10 gradients. The most outstanding protein spot was further analyzed by MALDI-TOF MS and protein database using the Mascot search engine. The protein was finally identified as APOAI (Apolipoprotein AI). Based on the known function of the APOAI, this gene acts protective action against the accumulation of platelet thrombin at the site of vascular damage for the pullorum disease. Therefore APOAI protein, identified in this study, can be a valuable biomarker in relation to the pullorum disease in chicken.

참고문헌 (Reference)

1 "Scaloni A 2003 Proteins from bovine tissues and biological fluids: defining a reference electrophoresis map for liver kidney muscle plasma and red blood cells" 440-3 460, 2003

2 "Probability based protein identification by searching sequ ence databases using mass spectrometry data" 3551-20 3567, 1999

3 "Mann M 2000 Proteomics to study genes and genomes" 837-405 846, 2000

4 "Kawai C 1988 Serum prostacyclin stabilizing factor is identical to apolipoprotein A-I" 803-807,

5 "Dernick R 1985 Simplified method for silverstaining of proteins in polyacrylamide gels and the mechanism of silver staining" 103-6 112,

6 "Bronzert TJ 1978 The amino acid sequence of human apoA-I an apolipoprotein isolated from high density lipoproteins" 623-630,

7 "Beynon RJ 2004 The proteome of chicken skeletal muscle: Changes in soluble protein expression during growth in a layer strain" 2082-4 2093, 2004