産褥期 感染 誘發 細菌의 生育을 억제하는 韓藥材 探索에 關한 硏究 = A study on screeining of antibacterial herb medicines against puerperal infecting-bacteria, Proteus vulgaris, Staphylococcus aures, and Enterococcus faecalis

국문 초록 (Abstract)

産褥期 感染이란 分娩後 生殖器의 細菌에 의한 感染을 意味하며, 産後 體溫上昇의 大部分은 骨盤感染에 의한 것으로 産褥期 感染의 重要한 指漂가 된다. 한의학에서 産後發熱의 病因病耭는 주로 邪毒感染, 血瘀, 外感, 血虛 등으로 보았으며, 치료는 一般的으로 産後疾患에는 處證이 많아 發表攻裏를 過度하게 하지 않고, 氣血과 營衛의 調和에 위주로 하나, 外感과 裏實證을 疏怒히 하면 안된다. 따라서 産後發熱을 惹起하는 各種 原因菌에 對한 客觀的인 抑制效果의 檢證은 産後發熱의 治療率을 높이는데 중요하다. 그 결과 산욕기감염을 유발하는 各種 細菌에 對해 黃連, 烏梅, 五味子 등의 水溶性 抽出物이 杭菌活性이 相對的으로 좋은 效果를 보였다. 따라서 産褥期 感染의 治療에 이들 韓藥材를 이용하면 보다 效果的일 것으로 생각된다.

다국어 초록 (Multilingual Abstract)

Deparment of Oriental Medicine, Graduate School of Dongguk University, Major in Oriental Ob & Gy.
Various kinds of medicinal herbs and prescribed herb medicines which have usually been used for treatment of reducing fever, purging intense heat, and detoxication were secreened for antibacterial activity against Proteus vulgaris, S. aureus, and E. faecalis which causing puerperal infection. Minimal inhibitory concentrations of water- and ethanol-soluble extracts of Hwangyon(C. japonica), Omae(P. mume), and Omiza(S. chinensis) were determined and using thin-layer chromatography the extracts of Hwangyon and Omiza were separated. The results obtained were as follows:
1. The water-soluble extracts of Keumeunhwa(L. japonica), Chiza(G. jasminoides), Hwangyon(C. japonica), Dansam(S. miltiorrhiza), Omae(P. mume) and Omiza(S. chinensis) showed antibacterial activities against P. vulgaris ; among them, the extracts of Hwangyon, Omae, and Omiza showed high antibacterial activities. The water-soluble extracts of Keumeunhwa(L. japonica), Hwangyon(C. japonica), Hwangkeum(S. baicalensis), Hwangbaek(P. amurense), Dansam(S. miltiorrhiza), Daehwang(R. coreanum), Omae(P. mume), Omiza(S, chinensis), Gingseng(P. ginseng), and Gamcho(G. uralensis) showed antibacterial activities against S. aureus ; among them, the extracts of Hwangyon and Omiza showed high antibacterial activities. The water-soluble extracts of Banggi(C. trilobus), Daehwang(R. coreanum), Omae(P. mume), Omiza(S. chinensis), and Gamcho(G. uralensis) inhibited the cell growth of E. faecalis ; among them, Omae and 0miza showed high antibacterial activities. The extract of Hwangyon(C. japonica) and Omae(P. mume) showed no inhibition against E. coli. However, the extract of Omiza(S. chinensis) inhibited cell growth of both E. coli and B. subtilis.
2. The water- and ethanol-soluble extract of Hwangyon(C. japonica) showed antibacterial activities against P. vulgaries and S. aureus, those of Omae(P. mume) against P. vulgaris and E. faecalis, and those of Omize(S. chinensis) against all species tested, P. vulgaris, S. aureus, and E. faecalis. With the exception that ethanol-soluble extract of Hwangyon(C. japonica) showed much higher antibacterial activity against S. aureus than water-soluble one, antibacterial activities of both water- and ethanol-soluble ones were similar to each of other two medicinal herbs.
3.When the prescribed herb medicines were tested, Sambohwan showed antibacterial activities against P. vulgaris and S. aureus. No prescribed herb medicine inhibited the cell growth of E. faecalis.
4. Minimal inhibitory concentrations(MICs) of both water- and ethanol-soluble extracts of Hwangyon (C. japonica), Omae(P. mume), and Omiza (S. chinensis) against P. vulgaris were 2.5㎎/㎖, 10㎎/㎖, and 20㎎/㎖, respectively. Those of both Hwangyon (C. japonica) and Omiza(S. chinensis) against S. aureus were 1.25㎎/㎖ and 10㎎/㎖, respectively. MICs of water-soluble extracts of Omae(P. mume) and Omiza(S. chinensis) aganist E. faecalis were 2.5㎎/㎖ and 5㎎/㎖, and those of ethanol-soluble extracts were 5㎎/㎖ and 10㎎/㎖, respectively. Except for those of E. faecalis, the cell growth of P. vulgaris and S. aureus were inhibited by much lower concentration of ethanol-soluble extracts used.
As a result, the antibacterial compounds against P. vulgaris, S. aureus, and E. aecalis are contained in the extracts of Hwangyon(C, japonica), 0mae(P. mume), 0miza(S. chinensis), the prescribed herb medicine, Sambohwan, and might be used for treatment of puerperal infection. Further study should be carried out to identify which compounds affect the cell growth inhibition of P. vulgaris, S. aureus, and E. faecalis.