가토 슬관절의 골연골 결손시 하이알유론산을 처리한 연골 세포 이식 후 조직-형태학적 결과 = Chondrocyte Transplantation with Hyaluronic Acid for Osteochondral Defect in the Rabbit's Patella

다국어 초록 (Multilingual Abstract)

Background : Regeneration of the damaged joint with osteochondral defects is an area of great interest because of the limited potential of defected articular cartilage healing. Recently, hyaluronic acid treated culture method was paid on attention for its advantage, increasing the number of chondrocytes and thus improving the quality of chondrocyte transplantation. The objective of this in vivo study was to determine the effect of chondrocyte transplantation with hyaluronic acid on the ability of regeneration.
Methods : Suspensions of rabbit articular chondrocyte were prepared from primary, high-density monolayer cultures for 2 weeks. After 14 days of culture, the cells were isolated by trypsinization and transplanted into chondral defects with an autogenous periosteal patch and hyaluronic acid. Articular cartilages were histologically examined at 4 weeks, 8 weeks after autogenic and allogenic chondrocyte transplantation with hyaluronic acid.
Results : Grafted defects were filled with cartilage in gross findings at 8 weeks. At 8 weeks, allogenic and autogenic chondrocyte transplantation significantly increased the amount of newly formed cartilage compared to that of only periosteal patch covered cartilage. Repaired tissue consisted of differentiated chondrocytes and hyaline cartilage.
Conclusion : These results suggest that hyaluronic acid provides a suitable environment for differentiation of chondrocytes and matrix production. Allogenic and autologous chondrocyte transplantation with hyaluronic acid may be clinically useful application in osteochondral defect of damaged joints.