만성간질환을 가진 환자에서 중합효소연쇄반응법 ( Polymerase Chain Reaction : PCR ) 을 이용한 HBV DNA 의 검출 = Detection of HBV DNA in Korean Patients with Chronic Liver Disiase using the Polymerase Chain Reaction ( PCR )

다국어 초록 (Multilingual Abstract)

Objectives: The detection of serum HBV DNA is important for the assessment of the disease activity and for the evaluation of the infectivity in chronic HBV infection. The goal of this study is to determine the detection rate of serum HBV DNA in Korean patients with chronic liver diseases by the PCR Southern blot analysis and to compare it with profiles of HBV serolosic markers. Methods: Serum samples from 306 Korean adults with chronic liver diseases(186 HBsAg(+ ) patients and 120 HRsAg( - ) patients)were tested for type B viral hepatits markers by RIA and HBV DNA by Polymerase Chain Reaction(PCR) Results : 1) In HBsAg(+ ) patients the detection rate of HBV DNA was 89.6%(164/186), Among these patients, in 96.1% of HBsAg(+), HBeAg(+)patients, 73.8% of HRsAg(+), anti-HBe (+)patiens, and 94.4% of HBsAg(+), HReAg( - ), anti- HBe( - )patients, HBV DNA was detected in their serum. 2) In HBsAg( - ) patients the detection rate of HBV DNA was 21,6%(26/120). Among these patients, 52.2% of anti-HBc(+), anti-HBs( - )patients, 13.0% of anti-HBc(+), anti-HBs(+)patients had HBV DNA in their serum. None of the HBsAg( - ), anti-HBc ( - ), anti-HBs( + ) patients had HBV DNA in their serum. Conclusion - HBV DNA was detected not only in HBsAg(+)patients but also in a significant portion(21.6%) Of HBSAg( - )patients, especially in anti-HBc(+)patients. Therefore hepatitis B virus may be related to HBsAg( - ) chronic liver diseases in Korea. Among anti-HBs(+)patients HBV DNA was detected in 13.0%, so that they may have some live virus even though they have obtained immunity against the virus. In 73.8% of HBsAg(+ ), anti-HBe(+ )patients, HBV DNA was deteced.