Optogenetic Engineering of Bar Domain Proteins [electronic resource]

다국어 초록 (Multilingual Abstract)

Nanoscale membrane curvature is understood to play an active role in essential cellular processes such as endocytosis, exocytosis, and actin dynamics. Few methods, however, can precisely manipulate membrane curvature in live cells. Making use of BAR domain proteins, a well-studied superfamily of membraneremodeling proteins, and the improved Light-Inducible Dimer (iLID) system, we developed a new method of generating nanoscale membrane curvature in live cells that is controllable, reversible, and capable of precise spatial and temporal manipulation. As proof of concept, we first engineered two optogenetic systems, opto-FBAR and opto-IBAR, that allow light-inducible formation of inward and outward membrane curvature, respectively. We then expanded upon this approach by engineering other BAR domain superfamily members to be light-inducible, including members of the FCHO, PACSIN, Amphiphysin, and NOSTRIN subfamilies. These systems present a novel approach for light-inducible manipulation of nanoscale membrane curvature in live cells.