The experiment was conducted to elucidate the differences of isozyme pattern and activity to esterase in susceptible and chlorpyrifos-(Rc), dichlorvos-(Rd) and permethrin-(Rp) resistant strains of female house flies(Musca domestica L.) using the metho...
The experiment was conducted to elucidate the differences of isozyme pattern and activity to esterase in susceptible and chlorpyrifos-(Rc), dichlorvos-(Rd) and permethrin-(Rp) resistant strains of female house flies(Musca domestica L.) using the methods of agar gel electrophoresis and filter paper test. And the results were summarized as follows.
1. Esterase isozyme showed eleven bands in the susceptible and three resistant strains by agar gel electrophoresis.
1) Esterase isozyme bands were Estα1β1, Estα2β2, Estα3β3, -Estα1β1 in the head, Estα1β1, Estα2β2, Estβ6, -Estα1β1, -Estα4β4 in the thorax and Estα1β1, Estα2β2, -Estα1β1, -Estα5β5 in the abdomen of the susceptible and resistant strains.
2) Estα5β5 was detected in all part(head, thorax, abdomen) of resistant strains, and Estα4β4 was not detected in the head and thorax of resistant strains.
3) -Estα2β2 showed more high activity in the thorax of Rc strain than the other strains.
4) Only -Estα3β3 was detected in all part of Rd strain.
5) -Estα2β2 was not detected in all part, and Estα3β3, Estα4β4, Estβ6 were not detected in the abdomen of Rp strain, however, those were detected in susceptible strain.
2. Esterase-α and -β activities appeared in the head, thorax and abdomen of susceptible and resistant strains by filter paper test.
1) Esterase-α showed more high activity than esterase-β.
2) Esterase-α activities were 1.15, 1.18 and 1.03 times in the head, 0.87, 1.18 and 0.89 times in the thorax and 0.82, 1.51 and 1.30 times in the abdomen of Rc, Rd and Rp strains to the susceptible strain, respectively.
3) Esterase-β activities were lower 0.66, 0.77, 0.70 times in the head, 0.84, 0.90, 0.90 times in the thorax of Rc, Rd and Rp strains than susceptible strain, but higher 1.19, 1.28, 1.25 times in the abdomen, respectively.