노랑초파리 발생과정에서 sta 유전자의 발현 양상에 관한 연구 = The study of expression pattern of sta gene during development of Drosophila melanogaster

다국어 초록 (Multilingual Abstract)

Drosophila stuvarista(sta) gene encodes the ribosomal protein D-p40, consisting of 270 amino acids. The D-p40 is highly conserved between such evolutionarily distinct organisms as human(63% identity) and hydra(58% identity) and has the effect of Minute phenotype such as other ribosomal proteins.
We identified the sta gene in the process of Drosophila cDNA library screening and analyzed the developmental expression pattern of sta gene in the transcription level using in situ hybridization. Each animal stage and tissues of 3rd instar larva and adults were showed ubiquitously expression pattern. These results were similar to other ribosomal proteins that represented Minute phenotype. Also, we expressed the 31kDa recombinant proteins of D-p40 using his-tag expression system in E. coli and purified by affinity chromatography. Immunoblot result of the developmental stages was showedthat strongly detection only the stage of embryo and pupa. This result suggested that the expression of sta gene were regulated in the level of post-translation modification ailed considered that sta gene had to other function during development as well as to function as a core ribosomal component.

목차 (Table of Contents)

• 서론
• 재료 및 방법
• 초파리 사육
• In situ hybidization
• 재조합 단백질 발현 및 분리
• Western blotting
• 결과 및 고찰