The author has investigated, using the polyvalent dysentery phage, the .effect_ of air on the phage inactivating activity of dye, especially crystal violet, and obtained the following results.
In the dark and fluorescent light, the inactivating acti...
The author has investigated, using the polyvalent dysentery phage, the .effect_ of air on the phage inactivating activity of dye, especially crystal violet, and obtained the following results.
In the dark and fluorescent light, the inactivating activity of dysentery phage
of crystal violet was markedly increased by the active aeration of air and the intensifying was almost same regardless of existence of the light. At this time, if the dye was pretreated in air, the appearance of phage inactivation was similar to that of the dye not pretreated in air.
The phage inactivating activity of crystal violet was also increased by shaking of the mixture contained the dye and phage.
While the phage was promptly, inactivated by introducing of air into the crystal violet-phage mixture, the phage was slowly inactivated when. introduce the nitrogen gas and the intensifying of phage inactivating activity was not so especially different from that of control tube.
The phage inactivating activity of crystal violet experimented under the active aeration of air became more strong at 37ºC than 4ºC and 20ºC, and also, the higher the dye concentration was, the more the phage inactivating activity became strong.
Methyl green, brom cresol purple and acid fuchsin did not inactivate the dysentery phage even though air was introduced. Bismark brown, however, could inacti-, vate the phage by introducing of air.
The reaction occurred between the crystal violet and dysentery phage was relatively stable and the phage inactivated by dye was not reactivated by sonic vibration and dilution with distilled water at 37°C. The oxygen was probably seemed to be very important for the action of crystal violet to inactivate the dysentery phage.