LC-MS/MS와 Ion Chromatography를 이용한 테트라민 분석 = Tetramine Analysis using Liquid Chromatography- Tandem Mass Spectrometry and Ion Chromatography

다국어 초록 (Multilingual Abstract)

As a first step toward identifying a new method for testing sea snail tissue for toxins, and thus prevent food poisoning due to the ingestion of contaminated snails, we measured the tetramine [(CH_3)_4N^+] contents of sea snails from the Korean coast using both liquid chromatography-tandem mass spectrometry (LC-MS/MS) and ion chromatography. For tetramine tested, good linearity (r^2 = 0.9996) was observed between the amounts in the injected samples and the peak areas of standard toxins, which ranged from 0.1 to 100 ng. The recovery (%) of tetramine from spiked tissue and mid-gut gland samples ranged from 84.0 to 95.3%. The quantitative results for tetramine using this method were in good agreement with the theoretical values. LC-MS/MS has both high sensitivity and selectivity, which makes it possible to measure trace quantities of tetramine in samples.

참고문헌 (Reference)

1 목종수, "한국 연안산 권패류의 tetramine 함량" 한국수산과학회 40 (40): 63-67, 2007

2 Kawashima Y, "Toxicity and tetramine contents of salivary glands from carnivorous gastropods" 43 : 385-388, 2002

3 Power AJ, "The seasonality and role of the neurotoxin tetramine in the salivary glands of the red whelk Neptunea antigua (L.)" 40 : 419-425, 2002

4 Jeon JK, "Tetramine contents of buccinidae in the Korean waters" 23 : 61-62, 1990

5 Tazawa T, "Study on tetramine content in salivary gland of sea snails(part Ⅱ)" 54 : 63-64, 2004

6 Shindo T, "Study on contents of tetramine in salivary gland, meat and internal organs of buccinid gastropods (mollusca)" 41 : 17-22, 2000

7 Fujii R, "Spectrophotometric determination of tetramine in carnivorous gastropods with tetrabromophenolphthalein ethyl ester" 33 : 237-240, 1992

8 Codex Alimentarius Commission, "Report of the Thirty-first session" 2008

9 KFDA, "Occurence of Food Poisoning (2002)" Korea Food & Drug Administration 23-, 2003

10 Kim JH, "Identification of tetramine, a toxin in whelks, as the cause of a poisoning incident in Korea and the distribution of tetramine in fresh and boiled whelk (Neptunea intersculpta)" 72 : 1935-1940, 2009

11 Park YH, "Fundamentals of Food Processing" HyungSeol Publish company 679-680, 1994

12 Skoog DA, "Fundamentals of Analytical Chemistry. 6th ed" Saunders College Publishing 714-, 1996

13 Kawashima Y, "Determination of tetramine in marine gastropods by liquid chromatography/electrospray ionization-mass spectrometry" 44 : 185-191, 2004

14 Shindo T, "Determination of tetramine in gastropods (mollusca) by ion chromatography and the effect of cooking" 41 : 11-16, 2000

15 Kungswan A, "Assay method for tetramine in carnivorous gastropods" 52 : 881-884, 1986

16 Zhao JY, "Analysis of tetramine in sea snails by capillary electrophoresis-tandem mass spectrometry" 781 : 555-564, 1997

17 Noguchi T, "A Pictorial Handbook of the Toxic Fisher Related to Food Hygiene" 1997