버섯 세균갈색무늬병균(Pseudomonas tolaasii)에 대한 길항세균 Pseudomonas azotoformans HC5의 대량배양을 위한 최적 배양조건

다국어 초록 (Multilingual Abstract)

This study was conducted to investigate optimum conditions for mass production of antagonistic microbes Pseudomonas azotoformans HC5. P. azotoformans HC5 is a potent biological control agent to control brown blotch disease caused by Pseudomonas tolaasii. This markedly showed the antagonistic activity against P. tolaasii, the most destructive pathogen of cultivated mushrooms. To define the optimum conditions for the mass production of the P. azotoformans HC5, we have investigated optimum culture conditions and effects of various nutrient source on the bacterial growth. The optimum initial pH and temperature were determined as pH 6.0 and 15oC, respectively. The optimal concentration of medium elements for the growth of pathogen inhibitor bacterium was determined as follows: 0.6% adonitol, 1.5% yeast extract, 0.8% NH4H2PO4, 5mM MgSO4, and 0.2% asparagine.

목차 (Table of Contents)

• 서 론
• 재료 및 방법
• 공시균주 및 배양
• 배양적온
• 초기 pH의 영향
• 영양원에 따른 길항미생물의 증식에 미치는 영향
• 결과 및 고찰
• 온도와 pH에 따른 영향
• 탄소원에 따른 영향
• 무기질소원에 따른 영향
• 유기질소원에 따른 영향
• 아미노산에 따른 영향
• 무기염류에 따른 영향
• 길항미생물 HC5의 최적 배양조건
• 적 요
• 감사의 말씀
• References

참고문헌 (Reference)

1 이찬중, "버섯 세균성갈색무늬병원균(Pseudomonas tolaasii)의 분비독소(tolaasin)를 저해하는 미생물 Pseudomonas sp. HC1" 한국균학회 41 (41): 248-254, 2013

2 이찬중, "버섯 세균성갈색무늬병원균(Pseudomonas tolaasii)의 독소 (tolaasin) 저해균 Pseudomonas sp. HC1의 대량배양을 위한 최적 배양조건" 한국버섯학회 12 (12): 35-40, 2014

3 이찬중, "버섯 세균갈색무늬병균(Pseudomonas tolaasii)에 항균활성을 가지는 미생물 Pseudomonas azotoformans HC5" 한국균학회 42 (42): 219-224, 2014

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