연령에 따른 흰쥐 위점막의 형태학적 연구 = Morphological Study on the Gastric Mucosa of the Aging Rat

다국어 초록 (Multilingual Abstract)

Age differences in the gastric mucosa have been studied in young(three month-old), adult(twelve month-old) and aged (thirty month-old) rats. Pieces of the tissue were taken from the junctional area of the fore and fundic-stomach, fixed in Karnovsky's fixative(phosphate buffer, pH 7.3) and post-fixed in 1% osmium tetroxide(phosphate buffer, pH 7.3). The sections 1μm in thickness were made on a LKB-V ultratome, and were stained with toluidine blue.
The mean thickness of epithelial layer in the gastric mucosa was determined by multiple measurements of the sections using an ocular micrometer on a AO photomicroscope. The numerical changes of the parietal, chief, surface epithelial and endocrine cells in the gastric mucosa were compared based upon the numbers in mucosa of 1.0mm width and 1μm thickness (Parietal Cell Index, PCI; Chief Cell Index, CCI; Surface Epithelial Cell Index, SCI; Endocrine Cell Index, ECI).
The results were as follow;
1. In the fore-stomach, the thickness of epithelial layer of the gastric mucosa is 24.5μm in young rats; 37.3μm in adult ones; and 42.7μm in old ones.
2. The nucleolar indices in the fore-stomach, by epithelial cells are increased during aging.
3. In fundic-stomach, PCI, CCI, SCI are reduced during aging.
4. ECI in the fundic-stomach are drastically increased during aging.