Screening for anti-inflammation and anti-oxidant effects of mongolian plant extracts

다국어 초록 (Multilingual Abstract)

The purpose of this study is to find anti-inflammatory candidates by screening sample of 40 Mongolian medicinal crops. All the samples were tested the anti-inflammatory activity, such as production of nitric oxide (NO) in lipopolysaccharide (LPS)-induced Raw264.7 macrophages. In addition, the anti-oxidant activity of all the samples were analyzed by DPPH and ABTS-free radical scavenging assays (inhibition ratio at 50 μg/ml concentration), total polyphenol, and total flavonoid contents. Of the 40 samples, the inhibition of NO production showed excellent efficacy in the order of Paeonia anomala L, Crataegus sanguinea Pall, Betulla phlatyphilla, Parnassia palustris L, and Veronica incana. However, when considering the NO inhibition rate and anti-oxidant effects totally, the top three species were Artemisia frigida Willd, Cotoneaster Мongolicus (Rosaceae), and Pinus sylvestris (Pinaceae). Especially, 3 samples were significantly suppressed NO production in a concentration-dependent manner in Raw 264.7 cells. Therefore, 3 samples were showed outstanding effects in the analysis of anti-oxidant activity, such as DPPH and ABTS free radical assays, total polyphenol, and total flavonoid contents. Taken together, these results suggest that Artemisia frigida Willd, Cotoneaster Мongolicus (Rosaceae), and Pinus sylvestris (Pinaceae) could alleviated the inflammatory response through NO suppression and anti-oxidant activity, and may be used for the prevention and treatment of inflammatory diseases including osteoarthritis. But, further research should be accomplished the efficacy and the underlying mechanism in vivo experiment to use safely.