Hair Growth Promoting Effect of Dermal Papilla Like Tissues from Canine Adipose-Derived Mesenchymal Stem Cells in Athymic Nude Mice

다국어 초록 (Multilingual Abstract)

The purpose of this study was to investigate the protein expression pattern and the in vivo trichogenicity of dermal papilla like tissues (DPLTs) which made from canine adipose-derived mesenchymal stem cells (ASCs) in athymic nude mice. Canine ASCs were isolated and cultured from adipose tissue, and differentiation was induced by culturing ASCs in dermal papilla forming media. DPLTs were embedded in collagen gel and their structural characteristics and protein expression were evaluated by hematoxylin and eosin stain and immunohistochemistry. Athymic nude mice were divided into two groups (control and DPLTs groups) and DPLTs were injected in skin wounds of mice in the DPLTs group. The trichogenicity of DPLTs was assessed by gross and histological evaluation for 30 days. The fate and the growth factor-secretion effect of DPLTs were evaluated by immunohistochemistry and Western blotting. DPLTs have a compact aggregated structure, form extracellular matrix and highly express the protein specific for dermal papillae, including ALP and versican. New hair follicle formation was remarkable in nude mice of the DPLTs group in gross findings and H&E stain. Vascularization was increased in the DPLTs group, which was the effect of vascular endothelial growth factor secreted by DPLTs in vitro and in vivo. These data suggest that engineered canine DPLTs have characteristics of dermal papillae and have a positive effect on hair regeneration by secreting growth factors.

목차 (Table of Contents)

• I. INTRODUCTION 1
• II. MATERIALS AND METHODS 3
• Isolation and cultivation of adipose derived mesenchymal stem cells 3
• Reconstruction of dermal papilla-like tissues 4
• Immunohistochemistry of DPLTs 5
• Alkaline phosphatase activity 6
• Preparation of the experimental animal model 7
• Evaluation of hair growth in gross and histological examination 8
• Western blotting 9
• Statistical analysis 10
• III. RESULTS 11
• Receonstruction of DPLTs 11
• Structure and protein expression of DPLTs 11
• In vivo hair inductivity 13
• The role of DPLTs in hair inductivity 18
• IV. DISCUSSION 20
• V. REFERENCES 26
• VI. ABSTRACT IN KOREAN 32