This experiment was carried out to investigate the production of exotoxin by Bacillus thuringiensis serotype 1 and 3ab, and its effects on mice and black goat kidney cells.
The difference of exotoxin productivity between Bacillus thuringiensis seroty...
This experiment was carried out to investigate the production of exotoxin by Bacillus thuringiensis serotype 1 and 3ab, and its effects on mice and black goat kidney cells.
The difference of exotoxin productivity between Bacillus thuringiensis serotype 1 and 3ab was hard to recongnize.
Micrococcus flavus was ascertained as a suitable test organism for determining the productivity of exotoxin by plate agar assay method. However Excherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa were not susceptible to the exotoxin.
The non-concentrated supernatant of the serotype 1 and 3ab from 48 hour-culture, administered orally, intraperitoneally, subcutaneously, nasally and intracerebrally, did not have any toxic effects on mice and this was the same case with the 5x concentrated supernatant. Black goat cells treated with 10% concentration of nonconcentrated supernatant of the serotype 1 and 3ab from 48 hour-culture failed to demonstrate any cytotoxic effects.