Surface Coating of Polytetrafluoroethylene with Extracellular Matrix and Anti-CD34 Antibodies Facilitates Endothelialization and Inhibits Platelet Adhesion Under Sheer Stress

다국어 초록 (Multilingual Abstract)

Expanded polytetrafluoroethylene (ePTFE) polymers do not support endothelialization because of nonconductive characteristics towards cellular attachment. Inner surface modification of the grafts can improve endothelialization and increase the long-term patency rate of the ePTFE vascular grafts. Here we reported a method of inner-surface modification of ePTFE vascular graft with extracellular matrix (ECM) and CD34 monoclonal antibodies (CD34 mAb) to stimulate the adhesion and proliferation of circulating endothelial progenitor cells on ePTFE graft to enhance graft endothelialization.
The inner surface of ECM-coated ePTFE grafts were linked with CD34 mAb in the presence of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS) solution and the physicochemical properties, surface morphology, biocompatibility, and hemocompatibility of the grafts were studied. The hydrophilicity of CD34 mAb-coated graft inner surface was significantly improved. Fourier transform infrared spectroscopy analysis confirmed ECM and CD34 mAb cross-linking in the ePTFE vascular grafts with our method. Scanning electron microscopy analysis showed protein layer covering uniformly on the inner surface of the modified grafts. The cell-counting kit-8 (CCK-8) assay confirmed that the modified graft has no obvious cytotoxicity. The modified graft showed a low hemolytic rate (0.9%) in the direct contact hemolysis test, suggesting the modification improved hemocompatibility of biopolymers. The modification also decreased adhesion of platelets, while significantly increased the adhesion of endothelial cells on the grafts. We conclude that our method enables ePTFE polymers modification with ECM and CD34 mAb, facilitates endothelialization, and inhibits platelet adhesion on the grafts, thus may increase the long-term patency rate of the prosthetic bypass grafts.

참고문헌 (Reference)

1 Yoder MC, "endothelium the origin of endothelial progenitor cells" 30 : 1094-1103, 2010

2 Catto V, "Vascular tissue engineering: recent advances in small diameter blood vessel regeneration" 2014

3 Chandy T, "Use of plasma glow for surface-engineering biomolecules to enhance bloodcompatibility of Dacron and PTFE vascular prosthesis" 21 : 699-712, 2000

4 Hoshi RA, "The blood and vascular cell compatibility of heparin-modified ePTFE vascular grafts" 34 : 30-41, 2013

5 Yoon CH, "Synergistic neovascularization by mixed transplantation of early endothelial progenitor cells and late outgrowth endothelial cells : the role of angiogenic cytokines and matrix metalloproteinases" 112 : 1618-1627, 2005

6 Jin G, "Surface modifying of microporous PTFE capillary for bilirubin removing from human plasma and its blood compatibility" 28 : 1480-1488, 2008

7 Tanaka M, "Study of blood compatibility with poly(2-methoxyethyl acrylate). Relationship between water structure and platelet compatibility in poly(2-methoxyethylacrylate-co-2-hydroxyethylmethacrylate)" 3 : 36-41, 2002

8 Melchiorri AJ, "Strategies and techniques to enhance the in situ endothelialization of small-diameter biodegradable polymeric vascular grafts" 19 : 292-307, 2013

9 Petersen S, "Site-selective immobilization of anti-CD34 antibodies to poly(L-lactide)for endovascular implant surfaces" 102 : 345-355, 2014

10 Sohn YS, "Site-directed immobilization of antibody using EDC-NHS-activated protein A on a bimetallic-based surface plasmon resonance chip" 19 : 051209-, 2014

11 Lamichhane S, "Responses of endothelial cells, smooth muscle cells, and platelets dependent on the surface topography of polytetrafluoroethylene" 104 : 2291-2304, 2016

12 Yoder MC, "Redefining endothelial progenitor cells via clonal analysis and hematopoietic stem/progenitor cell principals" 109 : 1801-1809, 2007

13 Bu X, "Properties of extracellular matrix-like scaffolds for the growth and differentiation of endothelial progenitor cells" 164 : 50-57, 2010

14 Moby V, "Poly(styrenesulfonate)/poly(allylamine)multilayers : a route to favor endothelial cell growth on expanded poly(tetrafluoroethylene)vascular grafts" 8 : 2156-2160, 2007

15 Lee JH, "Platelet adhesion onto wettability gradient surfaces in the absence and presence of plasma proteins" 41 : 304-311, 1998

16 Zhou F, "Peptidemodified PELCL electrospun membranes for regulation of vascular endothelial cells" 68 : 623-631, 2016

17 Raz O, "Pathways mediating the interaction between endothelial progenitor cells (EPCs) and platelets" 9 : e95156-, 2014

18 Avci-Adali M, "New strategies for in vivo tissue engineering by mimicry of homing factors for selfendothelialisation of blood contacting materials" 29 : 3936-3945, 2008

19 Chong DS, "Nanotopography and plasma treatment : redesigning the surface for vascular graft endothelialisation" 49 : 335-343, 2015

20 Freedman JE, "Molecular regulation of platelet-dependent thrombosis" 112 : 2725-2734, 2005

21 Albers M, "Meta-analysis of polytetrafluoroethylene bypass grafts to infrapopliteal arteries" 37 : 1263-1269, 2003

22 Asahara T, "Isolation of putative progenitor endothelial cells for angiogenesis" 275 : 964-967, 1997

23 Mead LE, "Isolation and characterization of endothelial progenitor cells from human blood" 2008

24 Danen EH, "Integrins in regulation of tissue development and function" 201 : 632-641, 2003

25 Avci-Adali M, "Induction of EPC homing on biofunctionalized vascular grafts for rapid in vivo self-endothelialization—a review of current strategies" 28 : 119-129, 2010

26 Rotmans JI, "In vivo cell seeding with anti-CD34 antibodies successfully accelerates endothelialization but stimulates intimal hyperplasia in porcine arteriovenous expanded polytetrafluoroethylene grafts" 112 : 12-18, 2005

27 Lin Q, "In situ endothelialization of intravascular stents coated with an anti-CD34 antibody functionalized heparin-collagen multilayer" 31 : 4017-4025, 2010

28 Liu H, "Improved hemocompatibility and endothelialization of vascular grafts by covalent immobilization of sulfated silk fibroin on poly(lactic-coglycolic acid)scaffolds" 12 : 2914-2924, 2011

29 Njatawidjaja E, "Hydrophilic modification of expanded polytetrafluoroethylene(ePTFE)by atmospheric pressure glow discharge(APG)treatment" 201 : 699-706, 2006

30 Ranjan AK, "Human blood vessel-derived endothelial progenitors for endothelialization of small diameter vascular prosthesis" 4 : 7718-, 2009

31 Grunkemeier JM, "Hemocompatibility of treated polystyrene substrates : contact activation, platelet adhesion, and procoagulant activity of adherent platelets" 41 : 657-670, 1998

32 Motlagh D, "Hemocompatibility evaluation of poly(diol citrate)in vitro for vascular tissue engineering" 82 : 907-916, 2007

33 Mihaly J, "FTIR and FT-Raman spectroscopic study on polymer based high pressure digestion vessels" 79 : 497-501, 2006

34 Abbott WM, "Evaluation and performance standards for arterial prostheses" 17 : 746-756, 1993

35 Chade AR, "Endothelial progenitor cells restore renal function in chronic experimental renovascular disease" 119 : 547-557, 2009

36 Caiado F, Dias S, "Endothelial progenitor cells and integrins : adhesive needs" Fibrogenesis Tissue Repair 4-, 2012

37 Hristov M, "Endothelial progenitor cells : mobilization, differentiation, and homing" 23 : 1185-1189, 2003

38 Cines DB, "Endothelial cellsin physiology and inthe pathophysiology of vascular disorders" 91 : 3527-3561, 1998

39 Pearson JD, "Endothelial cell function and thrombosis" 12 : 329-341, 1999

40 Wang YX, "Effects of the chemical structure and the surface properties of polymeric biomaterials on their biocompatibility" 21 : 1362-1373, 2004

41 Tseng DY, "Effects of amide and amine plasmatreated ePTFE vascular grafts on endothelial cell lining in an artificial circulatory system" 42 : 188-198, 1998

42 Vashist SK, "Effect of antibody immobilization strategies on the analytical performance of a surface plasmon resonance-based immunoassay" 136 : 4431-4436, 2011

43 Vartanian KB, "Distinct extracellular matrix microenvironments of progenitor and carotid endothelial cells" 91 : 528-539, 2009

44 Melchiorri AJ, "Contrasting biofunctionalization strategies for the enhanced endothelialization of biodegradable vascular grafts" 16 : 437-446, 2015

45 Stroncek JD, "Comparison of endothelial cell phenotypic markers of late-outgrowth endothelial progenitor cells isolated from patients with coronary artery disease and healthy volunteers" 15 : 3473-3486, 2009

46 Yin M, "Combinatorial coating of adhesive polypeptide and anti-CD34 antibody for improved endothelial cell adhesion and proliferation" 20 : 1513-1523, 2009

47 Walachova K, "Colonization of ion-modified polyethylene with vascular smooth muscle cells in vitro" 23 : 2989-2996, 2002

48 Nichols MD, "Coagulation-induced resistance to fluid flow in small-diameter vascular grafts and graft mimics measured by purging pressure" 101 : 1367-1376, 2013

49 Bacakova L, "Cell adhesion on artificial materials for tissue engineering" 53 : S35-S45, 2004

50 Ravi S, "Biomaterials for vascular tissue engineering" 5 : 107-128, 2010

51 de Mel A, "Biofunctionalization of biomaterials for accelerated in situ endothelialization : a review" 9 : 2969-2979, 2008

52 Shimizu T, "Arterial conduit shear stress following bypass grafting for intermediate coronary artery stenosis : a comparative study with saphenous vein grafts" 25 : 578-584, 2004

53 Chen JL, "Adsoption CD34 antibody onto the col/hep coating film on titanium to improve cytocompatibility of titanium" 47 : 1411-1414, 2008

54 Gong X, "Adhesion behavior of endothelial progenitor cells to endothelial cells in simple shear flow" 27 : 1071-1080, 2011

55 Goh ET, "Accelerating in situ endothelialisation of cardiovascular bypass grafts" 16 : 597-627, 2014

56 Williams SK, "Accelerated neovascularization and endothelialization of vascular grafts promoted by covalently bound laminin type 1" 99 : 67-73, 2011

57 Faries PL, "A comparative study of alternative conduits for lower extremity revascularization : all-autogenous conduit versus prosthetic grafts" 32 : 1080-1090, 2000

58 Larsen CC, "A biomimetic peptide fluorosurfactant polymer for endothelialization of ePTFE with limited platelet adhesion" 28 : 3537-3548, 2007